在灵长类动物中,克隆扩张和生殖中心和记忆B细胞对增强免疫的反应的多样化
Lachlan P Deimel1, Yoshiaki Nishimura2, Gabriela S Silva Santos1
1Laboratory of Molecular Immunology, The Rockefeller University, New York, NY, USA.
Cell reports
|August 13, 2025
概括
在灵长类动物中,刺激SARS-CoV-2 (严重急性呼吸系统综合征冠状病毒2) 的mRNA疫苗会触发记忆B细胞重新进入生殖中心 (GCs) 以进一步免疫发育,无论刺激地点如何.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
- 病毒学 病毒学
背景情况:
- 有效的疫苗诱导B细胞反应,通过生殖中心 (GC) 反应产生记忆B细胞和血细胞.
- 在小鼠中,记忆B细胞在增强时通常分化为血细胞,很少重新进入GCs.
- 灵长类循环记忆B细胞的比例比小鼠 (1%-2%) 大得多 (30%-50%).
研究的目的:
- 在严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) mRNA疫苗接种和增强后,研究 rhesus 的记忆和 GC B 细胞反应.
- 确定增强部位 (双侧面与对侧面) 对记忆B细胞区和抗体中和的影响.
- 为了阐明灵长类动物在二次抗原暴露时记忆B细胞的行为.
主要方法:
- rhesus 被免疫,然后用 SARS-CoV-2 mRNA 疫苗进行免疫增强.
- 与初始免疫接种相比,疫苗增剂在相同的部位 (双侧) 或不同的部位 (对侧) 进行.
- 进行了对记忆B细胞区块大小,抗体中和活性和GCB细胞参与度的分析.
主要成果:
- 记忆B细胞区的中和活性和大小不受增强部位的影响.
- 记忆B细胞在刚刚形成的GC中积极进入并经历了代扩张,当提升发生在远部位时.
- 这表明记忆B细胞在灵长类动物持续免疫反应中的动态参与.
结论:
- 在灵长类动物中,高亲和度的记忆B细胞作为持续免疫力的活跃储存库.
- 记忆B细胞可以重新进入生殖中心,并在增强后扩张,即使在遥远的解剖部位.
- 这与小鼠模型形成鲜明对比,并突出了物种之间的B细胞记忆动态的关键差异.
关键词:
B 细胞 B 细胞 B 细胞CP: 免疫学 免疫学这就是SARS-CoV-2病毒.抗体是对抗体的重要组成部分.发芽中心的发芽中心.在mRNA疫苗的使用中.记忆 B 细胞 B 细胞中和化 中和化 中和化非人类灵长类动物.疫苗增强剂是疫苗的增强剂.更多相关视频
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