昆虫圆顶受体直接识别病毒包裹以激活JAK/STAT信号,并诱导保存的抗病毒毒蛇蛋白表达
Siyu Chen1, Yupeng Tang1, Chunyu Zhang1
1State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
昆虫Janus酶/信号转换器和转录激活器 (JAK/STAT) 途径对于抗病毒防御至关重要. 胆矮人病毒的外囊蛋白与顶受体结合,启动JAK/STAT信号传递和毒蛇体表达,但Pns11蛋白阻止了这一点.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- 昆虫Janus酶/信号转换器和转录激活器 (JAK/STAT) 途径对于抗病毒防御至关重要.
- 树冠病毒激活这种途径的机制尚未完全理解.
研究的目的:
- 为了阐明树冠病毒如何激活昆虫JAK/STAT通路.
- 为了确定参与这个过程的病毒模式识别受体 (PRRs).
- 了解宿主抗病毒信号的病毒对抗性.
主要方法:
- 圆顶受体和RGDV外体蛋白的结构和突变分析.
- 研究病毒蛋白和宿主因子之间的蛋白质-蛋白质相互作用 (Dome,JAK,STAT).
- 评估viperin作为抗病毒有效剂的作用.
主要成果:
- 胆小矮病毒 (RGDV) 的外囊蛋白直接与顶受体结合,启动JAK/STAT通路的激活.
- 圆顶中的氨酸743对于病毒识别至关重要,识别圆顶作为病毒PRR.
- 维佩林是一种STAT调节的抗病毒效应剂,具有广泛的活性.
- RGDV非结构蛋白Pns11通过阻断STAT酸化和核转位,抑制viperin表达来对抗JAK/STAT信号传递.
结论:
- 叶圆顶作为病毒PRR,启动JAK-STAT信号,对抗病毒免疫至关重要.
- 像RGDV这样的 arboviruses 已经发展出可以对抗Dome-JAK-STAT通路的机制,从而导致持续感染.
- 这些发现揭示了昆虫病毒免疫逃避的新机制.
更多相关视频
06:44Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection
Published on: January 26, 2019
12:43Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE
Published on: July 29, 2014
相关概念视频
Immune Response Against Viral Pathogens
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
The JAK-STAT Signaling Pathway
Receptor Downregulation in MVBs
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
Viral Structure
Antigen Processing Pathways
MHC Class I: Presenting Endogenous...
Regulation of the Unfolded Protein Response
