追踪植物原酶的逐步达尔文进化过程
Colin Y Kim1,2,3,4, David W Kastner2,5, Andrew J Mitchell1
1Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Science advances
|August 13, 2025
概括
酶很少在植物中添加素,但Menispermaceae中的脱酸胺酶 (DAH) 是一个例外. 这项研究揭示了DAH通过重复和突变从醇合成酶 (FLS) 基因进化,说明了酶进化途径.
科学领域:
- 生物化学 生化学
- 进化生物学 进化生物学
- 植物新陈代谢 植物新陈代谢
背景情况:
- 生物化是植物中罕见的代谢过程.
- 脱甲胺原酶 (DAH),一种依赖铁和2氧酸的二氧化酶 (2ODD),是一个例外,催化了Menispermaceae中的甲生物合成.
- 了解从祖先形式进化的酶专业化对于代谢进化研究至关重要.
研究的目的:
- 调查脱酸胺酸酶 (DAH) 的进化起源和多样化.
- 阐明驱动酶新功能化的分子机制和进化途径.
- 了解酶如何通过达尔文进化来适应和切换催化机制.
主要方法:
- 用Menispermum canadense基因组进行了基因组测定和合成分析.
- 结构建模和分子动力学模拟.
- 位点定向突变发生,以确定关键的功能突变.
主要成果:
- DAH通过祖先的黄醇合成酶 (FLS) 基因的协同重复进化,随后是新功能化和基因丢失.
- 特定的突变被确定,使得从FLS到DAH活动的催化转换成为可能.
- 进化之路涉及到穿越健身山谷,中间状态保留在M. 加拿大人的基因组.
结论:
- 酶功能可以通过涉及基因重复和新功能化的谱系特定途径进化.
- 酶中的结构和功能转变是由重塑活性位点的特定突变驱动的.
- 这项研究提供了关于酶适应和催化机制切换的复杂进化格局的见解.
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