追踪肠杆菌,微生物组和抗生素耐药性基因,从废物到土壤,通过反复的堆肥应用
Syndia Sadikalay1, Laura Cavé2, Célia Ducat1
1Unit of Health and Environment, Institut Pasteur de Guadeloupe, Les Abymes, France.
PloS one
|August 13, 2025
概括
堆肥动物和人类废物减少了一些抗生素耐药的细菌和基因. 然而,对堆肥的反复应用重塑了土壤细菌群落,并影响了热带土壤中抗生素耐药性基因的丰富性.
科学领域:
- 环境微生物学环境微生物学
- 抗微生物耐药性 抗微生物耐药性
- 土壤科学 土壤科学
背景情况:
- 抗生素耐药细菌 (ARB) 和基因有助于抗生素耐药性增加.
- 通过动物便的环境污染可以传播ARB和抗生素.
- 来自废物的有机修正物在瓜德罗普被使用,但它们对耐药性的影响尚不清楚.
研究的目的:
- 评估堆肥和堆肥应用对瓜德罗普热带土壤中ARG和耐药肠道细菌的影响.
- 调查用于蔬菜生产的土壤中ARG和ARB的命运.
主要方法:
- 非培养性的方法来表征细菌群落和ARG含量.
- 培养方法来列举肠道细菌和表征耐药分离物.
- 耐药细菌的表型和基因型表征.
主要成果:
- 堆肥家禽便减少了埃舍里希亚和西格拉菌种群.
- 堆肥和堆肥应用改变了ARG的丰富性,有些增加,有些减少.
- 确定了可能参与传播抗微生物药物耐药性的细菌属.
结论:
- 堆肥原始废物对ARB和ARG水平产生影响.
- 连续的堆肥应用重新塑造了土壤肠杆菌群落,并影响了ARG的丰富性.
- 这项研究为热带农业土壤中耐药性传播提供了新的见解.
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