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通过DNA纳米结构进行CRISPR/Cas系统的精确传递,用于基因治疗和细胞内检测

Nachuan Song^1,2, Lijun Wang¹, Le Zhang¹

¹State Key Laboratory of Synthetic Biology, Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, P. R. China.

Chembiochem : a European journal of chemical biology

|August 13, 2025

概括

通过CRISPR/Cas基因编辑,可以通过Cas9,Cas12和Cas13蛋白进行精确的修改. DNA纳米结构显示出先进交付的前景,增强精密医学应用.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
生物工程是生物工程.

背景情况:

克里斯普尔/卡斯系统 (Cas9,Cas12,Cas13) 是功能强大的基因组编辑工具,具有多样化的应用.
临床翻译受到CRISPR/Cas组件高效和可控交付方面的挑战所阻碍.

研究的目的:

系统地审查CRISPR/Cas系统的当前交付方式.
强调基于DNA的功能材料作为先进的交付工具的作用.
探索DNA纳米结构的潜力,用于集成的超神论平台.

主要方法:

审查关于CRISPR/Cas传递系统的当前文献.
专注于基于DNA的功能材料和纳米结构.
对CRISPR/Cas系统和交付工具的集成策略的分析.

主要成果:

Cas9可实现精确的基因编辑,Cas12促进核酸检测,Cas13针对RNA进行治疗.
DNA纳米结构为先进的CRISPR/Cas交付提供了一个有前途的平台.
将CRISPR/Cas与DNA纳米结构相结合,可以产生超神论平台.

结论:

基于DNA的功能材料,特别是纳米结构,代表了CRISPR/Cas交付的重大进步.
开发使用CRISPR/Cas和DNA纳米结构的集成超神论平台,为精密医学带来了巨大的潜力.
进一步的生物工程方法对于实现CRISPR/Cas系统的全部临床潜力至关重要.

关键词:

这就是CRISPR/CasPR.在DNA纳米技术中.基因疗法基因疗法细胞内检测细胞内检测

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