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从工业废物中利用氨酶产生新型细菌菌株:一种生物处理和优化方法

J Beula Isabel¹, M Premalatha¹

¹Department of Energy and Environment, National Institute of Technology, Tiruchirappalli 15, Tamil Nadu, India.

International journal of biological macromolecules

|August 13, 2025

概括

这项研究将Bacillus clarus细菌分离出来,从土豆废物中产生高活性粉酶. 这表明了工业酶生产的可持续方法.

科学领域:

生物技术是生物技术.
工业微生物学工业微生物学

背景情况:

酶对于可持续的工业过程至关重要.
氨酶是一种关键的工业酶,具有多种应用.
利用像土豆加工废物 (PPW) 这样的废物流用于酶生产,提高了经济可行性.

研究的目的:

从土壤和PPW中分离和识别产生糖酶的细菌.
通过响应表面方法 (RSM) 来优化氨酶的产生.
评估白菌对于大规模,可持续的酶生产的潜力.

主要方法:

从土壤和PPW中分离细菌.
使用16S rRNA测序进行分子鉴定.
使用一次变量 (OVAT) 和RSM进行酶活性测定和优化.

主要成果:

鉴定了Klebsiella pasteurii NITTBP2和白菌 NITTBP1.1. 这两种植物.
清菌表现出明显更高的原始粉酶特异性活性 (136.15 单位/毫克),而不是K. pasteurii (7.69 单位/毫克).
在优化的条件下,在35°C,pH值为8和粉7g/L时,原始粉酶的最大特异活性为139.23U/mg,每公斤PPW的生产潜力为~5-6 × 10^5U/mL.

结论:

清杆菌是一种强大的细菌,用于工业粉酶生产.
PPW是可持续和经济高效的氨酸制造的可行基质.
这项研究通过废物回收来支持环保的酶生产.

关键词:

氨基酶是一种氨基酶.细菌明 NITTBP1 的一个.马加工废弃物马加工废弃物具体活动具体活动具体活动粉的水解,就是粉的水解.

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