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室内氧气度会影响线粒体功能和过氧化在透的人类骨肌纤维中的出现

Bradley A Ruple¹, Soung Hun Park², Jesse C Craig¹

¹Geriatric Research, Education, and Clinical Center, George E. Wahlen VA Medical Center, Salt Lake City, UT, United States of America; Department of Internal Medicine, Division of Geriatrics, University of Utah, Salt Lake City, UT, United States of America.

Biochimica et biophysica acta. Bioenergetics

|August 13, 2025

概括

高氧水平不会改变骨肌纤维中的最大线粒体呼吸. 然而,高氧度会增加过氧化,可能会损害亚最大呼吸.

科学领域:

运动生理学运动生理学
线粒体生物学线粒体生物学
生物化学生物化学

背景情况:

骨肌肉线粒体呼吸通常在高氧 (O2) 条件下测量ex vivo,以防止氧气限制.
高度的O2可以矛盾地增加活性氧物种 (ROS) 的产生,可能会影响线粒体功能.

研究的目的:

为了研究高与低的氧气部分压力 (PO2) 对线粒体呼吸和氧化物 (H2O2) 在透气化骨肌纤维中的生产的影响.
测试高PO2是否影响最大呼吸速率 (Vmax),次最大呼吸和累积H2O2外观.

主要方法:

利用高分辨率呼吸计来监测未经训练的参与者的透气化骨肌纤维中的线粒体状态3呼吸和H2O2出现率.
在控制下 (~127 mmHg) 和高 (~327 mmHg) PO2条件下的呼吸比较.

主要成果:

在高和控制PO2条件之间,最大状态3呼吸速率 (Vmax) 并没有显著差异.
在较低的PO2水平 (5-60 mmHg) 下的亚最大呼吸率在高的PO2下下降.
总累积H2O2的出现在高PO2下显著增加,并与下最大呼吸减少相关.

结论:

高度的氧气单独不会损害透的骨肌纤维的最大线粒体呼吸.
在高PO2下增加H2O2的产量可能会对亚最大线粒体呼吸功能产生负面影响.
这些发现突出了在确保氧气可用性和管理活体线粒体评估期间的氧化应激之间的潜在权衡.

关键词:

最大的呼吸时间.有氧气的可用性.有反应性氧物种的反应性氧物种.

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