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通过PacBio测序和Hi-C技术实现Onychostoma macrolepis的染色体级基因组组装

Lu Zhang¹, Ying Zhu¹, Lanzhu Zou²

¹School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China.

Scientific data

|August 13, 2025

概括

使用先进的测序技术组装了Onychostoma macrolepis的染色体级基因组. 这种高质量的基因组为保护这种有价值的商业鱼类物种提供了至关重要的资源.

科学领域:

基因组学就是基因组学.
水生生物学水生生物学
保护遗传学保护遗传学

背景情况:

巨 (Onychostoma macrolepis) 是一种具有商业价值的鱼类,具有很高的食用价值.
由于过度捕捞和环境污染,Onychostoma macrolepis的野生种群正面临急剧下降.

研究的目的:

为了组装一个高质量的,染色体水平的基因组Onychostoma macrolepis.
为保护和恢复Onychostoma macrolepis.提供基因组资源.

主要方法:

使用了一种综合方法,整合了Illumina测序,PacBio测序和Hi-C技术.
进行了基因组组装和注释,以确定蛋白质编码基因.

主要成果:

成功组装了一个944.02MB的染色体级基因组,用于Onychostoma macrolepis.
定了99.15%的组装序列到25个染色体上,具有32.36 Mb的N50结合.
预测了26,294个编码蛋白质的基因,其中96.30%的功能注释.

结论:

高质量的基因组组合为Onychostoma macrolepis研究提供了宝贵的资源.
这些基因组数据将支持保护和恢复Onychostoma macrolepis野生生殖质资源的努力.

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Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.