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Updated: Sep 11, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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基因组PAM指导使用哺乳动物基因组重复的CRISPR-Cas核酶的PAM特征和工程

Miao Yu1,2, Limei Ai1,2, Bang Wang2,3

  • 1Department of Biomedical Sciences and Tung Biomedical Sciences Centre, College of Biomedicine, City University of Hong Kong, Kowloon, Hong Kong SAR, China.

Nature biomedical engineering
|August 13, 2025
PubMed
概括

我们开发了GenomePAM,这是一种新方法来表征CRISPR-Cas原空间器相邻基因 (PAM) 在哺乳动物细胞中的偏好. 这种方法可以实现可扩展的发现Cas酶和基因编辑应用的工程变体.

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