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一种针对犬乳腺癌的小分子化合物通过CMT-N7中的基质子修饰来调节CXCL10和MECOM转录

Rongrong Wang^1,2, Chuyang Zhu³, Xiaoyue Yuan³

¹MOE Joint International Research Laboratory of Animal Health and Food Safety and Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.

Animals : an open access journal from MDPI

|August 14, 2025

概括

RORγ 抑制剂 W6134 和 XY018 在狗乳腺癌细胞中调节炎症和亡途径. 这些发现突出了RORγ作为一种潜在的治疗点,通过通过影响基因表达通过基因素修饰来治疗犬癌.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
兽医医学兽医医学兽医医学

背景情况:

核受体,包括RORγ,调节与炎症相关的基因,是癌症治疗的目标.
狗乳腺癌 (CMT) 是狗中普遍存在且危及生命的疾病,需要新的治疗策略.
RORγ在炎症中的作用表明它有可能成为CMT治疗的点.

研究的目的:

研究RORγ抑制剂W6134和XY018对犬乳腺癌细胞 (CMT-N7) 炎症基因表达的作用.
探索基因基因修饰在调解RORγ抑制剂对基因表达的影响中的作用.
为RORγ作为犬乳腺癌治疗点提供分子证据.

主要方法:

用RORγ抑制剂W6134和XY018.8对CMT-N7细胞进行治疗.
分析与炎症和亡相关的信号通路.
染色体免疫沉,然后进行定量PCR (ChIP-qPCR),以检测基因标记物在基因位置 (CXCL10,MECOM) 的丰富.

主要成果:

W6134和XY018治疗提高了炎症和亡信号通路的调节.
在CXCL10和MECOM基因位点检测到的基因修饰 (P300,H3K27ac,H3K4me1,H3K9la,H3K9bhb).
抑制RORγ影响了相关炎症基因的表达.

结论:

RORγ在狗乳腺癌中起着保护作用,可能是通过通过组织蛋白修饰来调节炎症途径.
RORγ 抑制剂 W6134 和 XY018 显示出作为治疗犬乳腺癌的治疗剂的潜力.
这些发现为改善乳腺癌犬的治疗结果和生存率提供了新的见解.

关键词:

在RORγ中,RORγ是指RORγ.狗的乳腺癌狗的乳腺癌基质子的修改基质子的修改这是一种炎症炎症炎症炎症.

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