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优化精液冷程序和氧化应激调节的优化

Zhicheng Wang¹, Haotian Gu¹, Chunhong Zhu¹

¹Jiangsu Institute of Poultry Science, Yangzhou 225125, China.

Animals : an open access journal from MDPI

|August 14, 2025

概括

优化水禽精液冷保存对于遗传保护至关重要. 一个分阶段的冷却程序 (P4) 显著改善了子精子的活力和运动性,通过减轻冷期间的氧化应激.

科学领域:

生殖生物学生殖生物学
低温生物学低温生物学
动物科学动物科学

背景情况:

水鸟精液冷保存对于遗传资源的保护和人工繁殖至关重要.
禽类精子在冷过程中容易受到氧化应激,从而降低了它们的生存能力.
独特的精子形态和生物化学特性有助于这种易感性.

研究的目的:

评估四种不同的冷方法对子精液质量的有效性.
为了比较这些程序对精液中抗氧化剂指数的影响.
为了确定水禽精液的最佳冷保存协议.

主要方法:

对子精液应用了四种不同的冷协议 (P1-P4).
结后的精子质量参数 (活力,运动性) 被评估.
对抗氧化剂指数的变化进行了监测,包括超氧化脱酶 (SOD),酶 (CAT),谷甲氧化酶 (GSH-px) 和甲 (MDA).

主要成果:

与其他协议相比,冷程序P4,一个分阶段冷却策略,在冷后显著提高了精子活力 (71.41%) 和运动率 (51.73%).
程序P4维持了SOD和CAT活动,同时仅略有降低了GSH-px,与P2不同,P2显著降低了所有抗氧化酶.
P4有效地平衡了细胞内脱水和冰晶的抑制,减少了反应性氧物种的积累和氧化应激.

结论:

阶段冷却策略 (P4) 在子精液冷保存方面优越,通过减轻氧化压力来保持精子质量.
结协议通过调节氧化应激通路,直接影响子精子质量.
这项研究为标准化水禽精液冷保存技术提供了理论基础.

关键词:

地着子的子脂质过氧化过氧化氧化应激是一种氧化应激.精液冷程序是如何进行的精液质量精液质量精液质量

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