相关实验视频

Updated: Sep 11, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

微RNA表达分析和化学抵抗性非小细胞肺癌中的生物途径

Chara Papadaki¹, Maria Mortoglou¹, Aristeidis E Boukouris^1,2

¹Laboratory of Translational Oncology, School of Medicine, University of Crete, 71500 Heraklion, Greece.

|August 14, 2025

概括

研究人员确定了六种在非小细胞肺癌 (NSCLC) 中被抑制的microRNAs (miRNAs),可以预测对西斯普拉丁化疗的反应. 这一发现为NSCLC患者的个性化治疗策略提供了潜在的生物标志物.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

在非小细胞肺癌 (NSCLC) 中,西斯普拉丁化疗的有效性可能会因DNA损伤修复机制受损而受到损害.
微RNAs (miRNAs) 是DNA损伤修复的关键调节者,并且正在作为NSCLC中基于的化疗反应的预测生物标志物进行研究.

研究的目的:

使用生物信息学方法在NSCLC中对基化疗的响应者和不响应者之间识别差异表达的miRNA.
验证从NSCLC患者的临床组织样本中识别的miRNAs的表达.

主要方法:

从基因表达综合 (GEO) 存储库中检索和分析了两个miRNA微阵列表达式数据集 (N=69名NSCLC患者).
微阵列分析的利用线性模型 (Limma) 包用于差异表达分析以识别差异表达的miRNAs.
在临床瘤和匹配的正常组织 (N=20) 上使用定量实时PCR (qRT-PCR) 验证的miRNA表达.

主要成果:

在响应者和不响应者之间鉴定出112个差异表达的miRNA;元分析揭示了24个持续改变的miRNA.
生存分析表明22个miRNA与NSCLC预后有显著关联.
一个六个miRNA签名 (miR-26a,miR-29c,miR-34a,miR-30e-5p,miR-30e-3p,miR-497) 被确定,在非响应者下调,并与DNA损伤修复途径 (p53,Hippo,PI3K,TGF-β) 相关.

结论:

在NSCLC中确定了六个被抑制的miRNA的签名.
这六个miRNA的签名显示了潜力作为一个预测生物标志物,在NSCLC患者中对西斯普拉丁的反应.
这些发现表明miRNAs在NSCLC的DNA损伤修复和化疗反应中起着至关重要的作用.

关键词:

生物信息学是一种生物信息学.化学电阻是一种化学电阻.西斯普拉丁 (cisplatin) 是一种制剂.这就是miRNAs.非小细胞肺癌的肺癌.

更多相关视频

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...