转录因子LjWRKY50 影响莉花调节的花在Lonicera japonica中的花持续时间
Yanfei Li1, Yutong Gan1, Guihong Qi1
1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Engineering Research Center of Chinese Medicine Resource, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
研究人员确定了Lonicera japonica的种类.
科学领域:
- 植物科学 植物科学
- 遗传学 是一个遗传学.
- 药用植物学 医学植物学
背景情况:
- 日本花 (Lonicera japonica) 是一种重要的药用草本.
- 花持续时间影响质量和经济价值,但其遗传控制不清楚.
- 手动采摘花需要了解影响花开放时间的因素.
研究的目的:
- 为了阐明控制Lonicera japonica中花持续时间的遗传机制.
- 确定控制花开放时间的关键基因和调节途径.
- 为了开发用于繁殖的分子标记物,改进了Lonicera japonica品种.
主要方法:
- 进行了种群结构分析和全基因组选择扫描.
- 分子实验包括双化酶记者分析和qRT-PCR.
- 在LjWRKY50中发现了一种功能变异,并转化为dCAPS标记物.
主要成果:
- 雅斯酸盐 (JA) 被证实是负调节剂,加速了芽的开放.
- 转录因子LjWRKY50被确定为花持续时间的致病基因.
- 已经证明LjWRKY50可以激活莉花酸盐生物合成基因LjAOS.
结论:
- LjWRKY50在通过莉花通路调节花持续时间方面发挥着至关重要的作用.
- 开发了基因和标记资源,用于Lonicera japonica的分子育种.
- 这些发现提供了对药用植物开花时间的遗传基础的见解.
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