植入黄瓜中维生素C介导的寒冷压力耐受性背后的生理和转录基因机制
Panpan Yu1,2, Junkai Wang1,2, Xuyang Zhang1,2
1School of Breeding and Multiplication, Sanya Institute of Breeding and Multiplication, Hainan University, Sanya 572025, China.
Plants (Basel, Switzerland)
|August 14, 2025
概括
外源维生素C (Vc) 应用通过增强抗氧化防御和调节基因表达来增强接种的黄瓜幼苗的寒冷耐受性. 150毫克L-1Vc的最佳度提高了对抗寒冷压力的生理弹性.
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 黄瓜 (Cucumis sativus L.) 对寒冷压力有很高的敏感性,这限制了其在低温环境中种植.
- 将黄瓜移植到耐寒的根茎上是一种提高低温弹性的策略.
- 外源的维生素C (Vc) 应用可能提供一种方法,以进一步提高植入植物的耐寒性.
研究的目的:
- 研究外源维生素C (Vc) 减轻植入黄瓜幼苗的寒冷压力的生理和分子机制.
- 为了确定最佳的Vc度以提高耐寒性.
- 阐明Vc在调节与压力反应和抗氧化剂防御有关的基因表达中的作用.
主要方法:
- 植入的黄瓜幼苗 (树苗:"Chiyu 505",根茎:"Chuangfan No.1") 通过叶子喷雾处理了不同度的Vc (50, 100, 150, 200 mg L-1).
- 幼苗经过3天的寒冷压力 (8°C),并继续使用Vc.
- 评估了形态,生理参数 (例如,叶绿素含量,抗氧化酶活性,可溶糖,素) 和基因表达 (转录组学,qRT-PCR).
主要成果:
- 150毫克的L-1Vc治疗显著减少了寒冷损伤,增加了根与芽的比率,根活力,叶绿素含量和抗氧化酶活动 (SOD,POD,CAT).
- 这种最佳Vc度也提高了可溶糖,可溶蛋白和蛋白水平,表明改善了减轻压力.
- 转录组分析显示,Vc调节了参与光合作用,压力信号转导 (例如MYC2,WRKY) 和抗氧化剂防御 (例如SOD) 的基因表达.
结论:
- 外源应用150毫克L-1维生素C有效地提高了植入的黄瓜幼苗的寒冷耐受性.
- Vc通过调节与光合作用,压力信号和抗氧化剂防御相关的生理反应和基因表达网络来提高抗寒能力.
- 这项研究表明了Vc作为生物刺激剂的潜力,用于在低温地区可持续种植移植黄瓜.
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