患有癌症的儿童发烧:使用血液转录组学的病理生理学见解
Lotte Møller Smedegaard1, Kia Hee Schultz Dungu1, Yuliu Guo2
1Department of Pediatrics & Adolescent Medicine, Copenhagen University Hospital, Rigshospitalet, 2100 Copenhagen, Denmark.
International journal of molecular sciences
|August 14, 2025
概括
患有癌症的儿童的发烧通常不清楚. 血液转录学揭示了感染与炎症的独特基因模式,有助于抗生素决定.
科学领域:
- 儿科瘤学 儿科瘤学
- 传染性疾病 传染性疾病
- 分子生物学分子生物学
背景情况:
- 在接受化疗的儿童中,发烧很常见,通常是由于感染或炎症引起的.
- 目前的诊断方法是不够的,导致长时间使用抗生素,即使有负的血液培养.
- 了解发烧的原因对于儿童癌症患者的适当治疗至关重要.
研究的目的:
- 通过血液转录学来研究患有血液性恶性瘤的儿童发烧的病理生理机制.
- 要区分发烧的传染性 (细菌性) 和非传染性原因.
- 为了确定指导抗生素治疗的潜在生物标志物.
主要方法:
- 一项全国性的研究涉及168例患有血液性恶性瘤的儿童的发烧发作.
- 在PAXgene管中收集的全血样本用于单细胞RNA测序.
- 发烧儿童与没有细菌血症之间的转录形状的比较.
主要成果:
- 患有细菌病的儿童表现出与免疫调节,心脏和血管功能相关的基因的差异表达.
- 没有细菌病的儿童表现出明显的基因表达模式,表明病毒或其他炎症原因.
- 叠加的差异表达基因与先前建立的基于转录学的签名被确定.
结论:
- 血液转录组学为儿童癌症患者的发烧机制提供了新的见解.
- 独特的基因表达模式可以表明病毒感染或非细菌性炎症的孩子与阴性血培养.
- 这些发现支持在发烧的癌症儿童中早期停止抗生素治疗,当感染未被证实时.
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