在草药市场揭露伪造:MassARRAY iPLEX测试用于准确识别Plumbago indicaL
Kannika Thongkhao1,2, Aekkhaluck Intharuksa3, Ampai Phrutivorapongkul3
1School of Languages and General Education, Walailak University, Nakhon Si Thammarat 80160, Thailand.
International journal of molecular sciences
|August 14, 2025
概括
一个新的MassARRAY iPLEX测定准确地识别了Plumbago indica根,防止了草本产品中的改. 这种分子工具可以将P. indica与相关物种区分开来,确保治疗疗效和产品完整性.
科学领域:
- 药理学是指药理学,即药理学是指药理学.
- 分子生物学分子生物学
- 草药验证 验证 草药验证
背景情况:
- 普隆巴哥印加根是传统泰国多草药配方中的重要组成部分.
- 密切相关的物种,P. zeylanica和P. auriculata,可以改P. indica,可能降低治疗效果.
- 准确识别P. indica对于保持草药产品的质量和有效性至关重要.
研究的目的:
- 开发一种可靠的分子方法来区分Plumbago indica与其密切相关的物种.
- 确保在草药配方中使用的Plumbago indica原料的真实性.
- 为了防止在商业草药市场中改和错误识别.
主要方法:
- 开发一个MassARRAY iPLEX试验,利用内部转录间隔器 (ITS) 区域的五个单核酸多态 (SNP) 位点.
- 选择特定的SNP位置 (18,112,577,623和652) 作为物种识别的遗传标记.
- 用不同的DNA度和混合物种样本测试试验的准确性和灵敏度.
主要成果:
- 在MassARRAY iPLEX测定准确地确定了Plumbago indica,区分它从P. zeylanica和P. auriculata.
- 该试验检测到P. indica的DNA度低至0.01 ng/μL.
- 商业原始药物样本被发现含有P. zeylanica,表明潜在的伪造. 该试验成功地在混合样本中识别了P. indica.
结论:
- 开发的MassARRAY iPLEX测定是一种可靠和有效的分子工具,用于验证Plumbago indica的真实性.
- 这种测试可以防止错误识别和改P. indica原材料.
- 这种测定方法的应用对于确保草药产品的完整性和治疗价值至关重要.
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