通过酶性水解将番茄工业的副产品转化为抗真菌
Davide Emide1, Lorenzo Periccioli1,2, Matias Pasquali1
1Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, Via G. Celoria 2, 20133 Milan, Italy.
International journal of molecular sciences
|August 14, 2025
概括
番茄种子蛋白质被水解以产生生物活性. 布罗梅林处理的体对虫类物种表现出强烈的抗真菌活性,为农业食品副产品的价值化提供了可持续的方法.
科学领域:
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
背景情况:
- 农业食品副产品,如番茄种子,富含蛋白质和生物活性化合物.
- 这些副产品的价值化支持循环经济原则和可持续实践.
研究的目的:
- 开发一种用于从番茄种子中回收高蛋白质的生物化学过程.
- 为了优化酶化水解用于生成.
- 描述和评估它们的抗真菌性质.
主要方法:
- 性提取番茄种子蛋白质的方法.
- 使用胺素,帕帕因和胰腺素进行酶性水解.
- 通过SDS-PAGE,SEC-HPLC和RP-HPLC进行型分析.
- 在体外抗真菌测定对*Fusarium*物种.
主要成果:
- 布罗梅林化物产生了多样化的形状,并表现出显著的DPPH基因清除活性.
- 所有的水解剂都表现出抗氧化特性.
- 布罗梅林衍生可以抑制 *Fusarium graminearum* 和 *F. oxysporum* f.sp. 的发生. 在剂量依赖的方式以0.1 mg/mL的*lycopersici*的方式.
结论:
- 番茄种子蛋白的酶化水解是生产生物活性的可行策略.
- 这些具有有前途的抗真菌活性,可用于潜在的农业应用.
- 这项研究有助于可持续发展的生物技术和食品工业废物的利用.
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