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Updated: Sep 11, 2025

Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

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在生物入侵期间,Bemisia tabaci的热耐性发生了与DNA甲基化相关的表观遗传变化

Tianmei Dai^1,2, Yusheng Wang², Xiaona Shen²

¹Key Laboratory of Forest Bio-Resources and Integrated Pest Management for Higher Education in Hunan Province, College of Forestry, Central South University of Forestry and Technology, Changsha 410004, China.

International journal of molecular sciences

|August 14, 2025

概括

全球变暖可能有利于入侵物种. DNA甲基化变化,而不是遗传变异,解释了为什么入侵的Bemisia tabaci (MED) 比土著的亚洲II3更能忍受热量,提供了新的控制目标.

科学领域:

生态生态学生态生态学
进化生物学进化生物学
遗传学遗传学是一个

背景情况:

全球变暖和气候变化正在增加外热物种的范围和丰富性,可能会加剧生物入侵.
像DNA甲基化这样的表观遗传修饰可能会将入侵物种的表型可塑性与温度变化联系起来.

研究的目的:

在入侵性 (Bemisia tabaci MED) 和土著性 (Bemisia tabaci AsiaII3) 物种中研究不同耐热性背后的表观遗传机制.
为了确定DNA甲基化是否影响这些物种的热电阻.

主要方法:

评估MED和AsiaII3在热和冷压力下的热承受能力.
使用光标记的甲基化敏感放大多态 (F-MSAP) 来检测DNA甲基化变化.
采用定量实时聚合酶链反应 (qRT-PCR) 和西式涂抹来分析DNA甲基转移酶 (Dnmt) 表达.

主要成果:

侵袭性MED物种在热应激下表现出比本土亚洲II3物种更高的耐热性.
F-MSAP分析表明,MED的热耐受性增加与DNA甲基化变化有关,而不是遗传变异.
在女性MED成年人中,Dnmt3的表达增加与更高的耐热度相关.

结论:

基因甲基化增强了入侵物种的热耐受性,为适应全球气温上升提供了适应机制.
通过Dnmt调节热耐受性对于理解入侵物种的成功和适应至关重要.
这项研究确定了在全球变暖的背景下控制入侵物种的潜在目标.

关键词:

贝米西亚烟草 (Bemisia tabaci) 是一种烟草.通过DNA甲基化.在DNA甲基转移酶中.这是生物入侵.快速适应快速适应耐热性耐热性耐热性

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