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通过协同机械细胞破坏和性溶解,从氏原体中提取增强的蛋白质

Jun Wei Ng¹, Sze Ying Lee¹, Tong Mei Teh¹

¹Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology, and Research (A*STAR), 31 Biopolis Way, Nanos, Singapore 138669, Singapore.

Foods (Basel, Switzerland)

|August 14, 2025

概括

高压均质化与温和的性处理相结合,显著增加了微藻从湿生物质中提取蛋白质. 这种综合方法提高了产量,同时减少了可持续蛋白质生产的浪费和能源使用.

科学领域:

食品科学食品科学食品科学
生物技术是生物技术.
可持续化学可持续化学

背景情况:

微藻蛋白对于食品和营养品有价值.
性处理对于提取蛋白质是常见的,但单独的治疗往往是不够的.
工业应用需要高效的提取方法.

研究的目的:

研究一种完整的方法,用于增强微藻蛋白质提取.
评估高压均质化 (HPH) 和性处理的协同效应.
使用湿生物质优化蛋白质产量,并最大限度地减少对环境的影响.

主要方法:

单独对*Auxenochlorella protothecoides*生物质进行性处理的测试.
应用单通HPH (35k psi) 湿生物质和喷雾干燥生物质.
结合HPH和轻度性处理 (0.02-0.1 M NaOH) 使用湿和喷雾干燥的生物质.
在各种条件下评估蛋白质提取产量.

主要成果:

仅用性处理就能产生最多2.4%的蛋白质.
仅HPH就能使52.3%的蛋白质从湿生物质中溶解,而喷雾干燥生物质的蛋白质为18.5%.
结合HPH和0.1M NaOH,从喷雾干燥的生物质中获得68.0%的产量.
使用湿生物质可以将NaOH的需求降低5倍 (0.02M),获得相似的产量 (68.1%).

结论:

对HPH和轻度性处理的协同应用对于微藻蛋白质提取非常有效.
利用湿生物质可显著降低的使用量,并消除了能源密集的干燥.
这种综合工艺显示了工业规模,可持续的微藻蛋白质生产的巨大潜力.

关键词:

性溶解的性溶解化提取提取提取提取高压均化高压均化机械细胞的破坏破坏.微藻是一种微藻.处理湿生物质的过程.

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