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Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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相关实验视频

Updated: Sep 11, 2025

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Published on: May 20, 2013

一个集群自适应曝光时间选择方法,用于HDR结构光3D重建.

Zhuang Li¹, Rui Ma¹, Shuyu Duan²

¹Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.

Sensors (Basel, Switzerland)

|August 14, 2025

概括

本研究引入了适应性曝光时间方法,用于边缘投射特征测量 (FPP),以改进高动态范围对象的3D测量. 新方法通过优化曝光设置和阶段解封策略,显著减少测量误差.

关键词:

暴露融合选择选择暴露融合边缘投影的概况测量方法高动态范围的高动态范围.

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相关实验视频

Last Updated: Sep 11, 2025

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Published on: May 20, 2013

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

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Published on: February 8, 2014

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

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Published on: May 30, 2016

科学领域:

* 光学和光子学 * 光学和光学
* 计量学和测量科学计量学和测量科学
* 计算机视觉和图像处理

背景情况:

*边缘投射型度测量 (FPP) 是工业中一个关键的非接触式3D测量技术.
*高动态范围对象对FPP构成挑战,导致相位计算错误.
* 现有的FPP方法在物体显示显著反射率变化时遇到困难.

研究的目的:

* 开发一种适应性暴露时间选择方法,用于FPP.
* 为了提高高动态范围对象的相位计算精度.
*为了减少工业3D计量中的测量误差.

主要方法:

* 改进的集群方法基于反射性对区域进行细分.
* 适应性暴露时间选择优化了暴露的数量和持续时间.
* 在阶段解封过程中采用阶段顺序共享策略.
* 互补的灰色代码模式用于跨暴露的相位顺序计算.

主要成果:

* 拟议的方法显著减少了测量误差.
*实验结果显示,测量误差减少了25.4%.
* 通过可比的总曝光时间来实现误差减少.
* 提高了对具有挑战性的物体的相位计算精度.

结论:

* 适应性曝光时间选择方法有效地解决了高动态范围对象的FPP限制.
* 这种技术提高了工业3D测量的精度和可靠性.
* 阶段顺序共享策略进一步有助于准确的阶段解封.
* 该方法为改善复杂场景中的FPP性能提供了实用解决方案.