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用内密封剂或氧化物处理的多种生物膜:抗菌活性和社区成分的变化

Steven K Uttech¹, Ronald Ordinola-Zapata¹, W Craig Noblett¹

¹Division of Endodontics, Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, USA.

International endodontic journal

|August 14, 2025

概括

氧化和BC Sealer有效地降低了根管生物膜中的细菌活力和DNA. AH Plus改变了生物膜的组成,但没有减少DNA,而没有消除多种生物膜.

科学领域:

微生物学微生物学
牙周内科医院牙周内科医院牙周内科医院
生物材料是一种生物材料.

背景情况:

根通道密封剂和氧化用于消除内感染.
评估它们的抗微生物活性和对生物膜社区组成的影响对于内牙治疗成功至关重要.

研究的目的:

评估AH Plus和BC Sealer与氧化相比对多种根运河生物膜的抗微生物疗效.
为了研究由这些内牙材料引起的细菌群体组成的变化.

主要方法:

在CDC的生物膜反应器中,牛牙筒被用牙斑接种.
测试的材料包括AH Plus,BC Sealer,氧化和对照材料 (甘醇和未经处理的).
使用殖民地形成单位 (CFU) 和定量PCR (qPCR) 评估了抗菌活性,同时通过下一代测序分析了社区组成.

主要成果:

与对照组相比,氧化和BC Sealer显著降低了CFU和qPCR值.
AH Plus降低了细菌的活力,但没有显著降低DNA度.
所有测试材料都改变了整体生物膜社区组成,AH Plus与未经处理的对照组相比显示出显著差异.

结论:

氧化和BC Sealer对根运河生物膜具有显著的抗菌作用.
AH Plus改变了生物膜社区结构,但对总体细菌负载的影响较小.
没有一个被评估的材料能够完全消除多种生物膜.

关键词:

牙内科的密封者密封者内治疗药物内药物微生物组是一个微生物组.下一代测序的下一代测序.

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