Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Introducing quality by design approach in early process development to improve natural killer cell manufacturing robustness.

Cytotherapy·2026
Same author

Viable cell density as an indicator for dynamic feeding strategy in fed-batch and perfusion CHO cell culture.

Scientific reports·2025
Same author

Evaluation of several type of chemical cryoprotectants and combination towards cryopreservation of NK cell.

Cytotechnology·2025
Same author

Development of a cognitive function assessment tool based on psychomotor function tests in patients with schizophrenia.

Schizophrenia research. Cognition·2025
Same author

3D Printing via Melt Extrusion Deposition Facilitates the Use of Extended-Release Profiles in Preclinical Research and Development.

Molecular pharmaceutics·2025
Same author

[A Case of Prolonged Survival of a Patient with Gastric Cancer Despite the Development of Pembrolizumab-Induced Fulminant Type 1 Diabetes Mellitus].

Gan to kagaku ryoho. Cancer & chemotherapy·2025

相关实验视频

Updated: Jun 25, 2026

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
10:44

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

Published on: February 2, 2011

48.7K

对 NK 细胞进行可扩展的生产工艺开发,以大规模扩张为目标.

Takuya Kikuchi1, Ippei Takeuchi1, Hideto Yamaguchi1

  • 1CMC Development, Chemical & Biological Labs, Astellas Pharma, Inc., 5-2-3, Tokodai, Tsukuba-shi, Ibaraki, 300-2698, Japan.

Regenerative therapy
|August 14, 2025
PubMed
概括

我们开发了一种可扩展的化生物反应器工艺,用于扩大诱导多能干细胞衍生的自然杀手 (NK) 细胞. 这种方法实现了1000倍的扩展,质量始终如一,为现成的NK细胞疗法铺平了道路.

科学领域:

  • 免疫学 免疫学 免疫学
  • 生物技术是生物技术.
  • 细胞疗法细胞疗法

背景情况:

  • 自然杀手 (NK) 细胞对癌症免疫疗法具有前景,因为它们降低了移植对宿主疾病 (GVHD) 的风险.
  • 目前的NK细胞扩张方法使用扩展策略,阻碍了"现成"应用.
  • 诱导多能干细胞 (iPSC) 衍生NK细胞的可扩展生物反应器培养仍未得到充分研究.

研究的目的:

  • 开发和评估一种可扩展的混合生物反应器工艺,用于iPSC衍生的NK细胞扩张.
  • 评估重复刺激和扩张对NK细胞产量和质量的影响.
  • 为了证明从实验室扩大NK细胞生产到试点规模的可行性.

主要方法:

  • 开发了一种用于iPSC衍生的NK细胞扩张的培养系统.
  • 采用用激素抗体和扩张NK细胞刺激的重复循环.
  • 评估了使用气化生物反应器的扩大参数,从1L转变为10L.

主要成果:

  • 与静态培养相比,杂培养显著增强了NK细胞扩张.
  • 通过重复循环保持质量,实现了iPSC衍生的NK细胞的1000倍扩张.
  • 成功将生产规模扩大到10升生物反应堆,产生可比的细胞扩张和质量.
关键词:
有空气的动生物反应器自然杀手细胞是什么?可扩展的过程可扩展的过程.

更多相关视频

Natural Killer NK and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line
11:02

Natural Killer NK and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line

Published on: February 8, 2022

5.8K
Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics
08:50

Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics

Published on: August 16, 2024

862

相关实验视频

Last Updated: Jun 25, 2026

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
10:44

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

Published on: February 2, 2011

48.7K
Natural Killer NK and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line
11:02

Natural Killer NK and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line

Published on: February 8, 2022

5.8K
Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics
08:50

Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics

Published on: August 16, 2024

862

结论:

  • 展示了一个可扩展的气化动生物反应器工艺,用于iPSC衍生的NK细胞扩张.
  • 优化的过程支持稳健的细胞扩张和适合治疗应用的质量.
  • 这种可扩展的方法促进了"现成"NK细胞疗法的临床转化.