相关实验视频

Updated: May 5, 2026

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

鼠标和人类胚胎基因组激活在单细胞阶段开始

Maki Asami¹, Anthony C F Perry¹

¹Laboratory of Mammalian Molecular Embryology, Department of Life Sciences, University of Bath, Bath, United Kingdom.

Frontiers in cell and developmental biology

|August 14, 2025

概括

胚胎基因组激活 (EGA) 开始比以前想象的更早,从小鼠和人类的单细胞阶段开始. 这种直接的EGA (iEGA) 涉及母亲和父亲的基因表达,对发育和癌症有影响.

科学领域:

发展生物学发展生物学
基因组学就是基因组学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

在受精时,受精性偶体在转录上保持沉默.
胚胎基因组激活 (EGA) 在早期胚胎中启动基因表达.
以前的理解将EGA置于后期的发育阶段 (小鼠有2个细胞,人类有4-8个细胞).

研究的目的:

研究早期胚胎基因表达的精确时间和性质.
为了确定EGA是否在单细胞阶段启动.
探索转录因子 (如MYC/c-Myc) 在早期发育中的作用.

主要方法:

鼠标和人类单细胞胚胎的单细胞RNA测序 (scRNAseq).
基因表达的时间过程分析.
功能性研究涉及阻断小鼠胚胎中的MYC/c-Myc活性.

主要成果:

有证据表明,EGA在小鼠和人类的单细胞阶段 (即时EGA,iEGA) 发起.
iEGA涉及在受精后不久的母体和父体基因组转录.
MYC/c-Myc转录因子对iEGA至关重要,它们的抑制会导致发育停止;c-Myc的抑制显示出广泛的基因抑制 (胚胎基因组抑制,EGR).

结论:

立即EGA (iEGA) 是发生在单细胞阶段的关键早期发育事件.
iEGA与以后的"主要EGA"不同,涉及关键的监管转录因素.
了解iEGA对发育过程,表观遗传遗传,干细胞研究和癌症生物学都有影响.

关键词:

胚胎基因组激活 (EGA) 的方法胚胎基因组抑制 (EGR) 的研究.这是受精,受精,受精.立即使用EGA.一个单细胞胚胎.一个单细胞RNA测序的序列.转录转录是一种转录.囊胞基因组激活 (ZGA) 是一种

更多相关视频

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Published on: June 14, 2024

相关概念视频

Cleavage and Blastulation

Cleavage and Blastulation

After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.

Gastrulation

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...

Zygotic Development And Stem Cell Formation

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...