相关实验视频

Updated: Sep 11, 2025

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Published on: June 7, 2019

在黑色素瘤细胞中,SETD6调解了BRD4和MITF的选择性相互作用和基因组占用

Tzofit Elbaz Biton^1,2, Michal Feldman^1,2, Tomer Davidy^1,2

¹The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben-Gurion University of the Negev, P.O.B. 653, Be'er-Sheva 84105, Israel.

|August 14, 2025

概括

SETD6通过修改关键蛋白质BRD4来调节黑色素瘤. 这种SETD6-BRD4相互作用,与MITF一起,控制黑色素瘤细胞中的基因转录.

科学领域:

分子生物学分子生物学
癌症研究癌症研究
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

异常转录驱动黑色素瘤,这是最致命的皮肤癌.
氨酸甲基转移酶SETD6在黑色素瘤中的作用尚不清楚.
通过在K99中甲基化BRD4,SETD6调节转录,影响mRNA转化基因.

研究的目的:

研究SETD6在黑色素瘤中的功能.
阐明黑色素瘤细胞中SETD6介导调节的机制.
确定与黑色素瘤病变发生有关的新型相互作用和途径.

主要方法:

研究了黑色素瘤细胞中的SETD6和BRD4相互作用.
使用SETD6和BRD4.4的淘汰和点突变模型.
使用ChIP和共免疫沉分析了基因组占用率和蛋白质相互作用.

主要成果:

在黑色素瘤细胞中发生在K99的SETD6介导的BRD4甲基化.
SETD6淘汰赛或BRD4-K99突变破坏了BRD4基因组结合.
SETD6与MITF相互作用,影响其基因组分布,并与BRD4.4形成一种新的染色质复合体.

结论:

在黑色素瘤的转录调节中,SETD6起着至关重要的作用.
一种涉及SETD6,甲基化BRD4和MITF的新型染色体复合物对黑色素瘤至关重要.
这种复合体调解了BRD4和MITF在黑色素瘤中的特定基因组部位的选择性招募.

更多相关视频

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

Published on: March 8, 2022

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

Published on: November 13, 2012

相关概念视频

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

MAPK Signaling Cascades

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...