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多光谱光声学显微镜是由气体填充的空心纤维激光脉冲驱动的
Cuiling Zhang1, Marcello Meneghetti1,2, J E Antonio-Lopez3
1DTU Electro, Technical Universisty of Denmark, DK-2800 Kgs. Lyngby, Denmark.
Biomedical optics express
|August 14, 2025
概括
一种新的充满的纤维激光器为多光谱光声显微镜 (MS-OAM) 提供了多种光谱线. 这一突破使得通过针对近红外光谱中的关键生物分子,实现高分辨率,无标签的生物成像.
科学领域:
- 生物医学光学 生物医学光学
- 激光物理 激光物理
- 生物光子学 生物光子学
背景情况:
- 多光谱光声显微镜 (MS-OAM) 需要复杂的光源来精确匹配生物分子吸收.
- 现有的光源往往缺乏先进的MS-OAM应用所需的光谱灵活性和强度.
研究的目的:
- 为近红外 (NIR) MS-OAM开发和描述一种新的气体充满的反共振空心纤维 (ARHCF) 激光源.
- 为了证明这种激光源能够产生与关键生物分子吸收带精确对齐的多个光谱线的能力.
主要方法:
- 使用了一种充满 (H2) 的ARHCF激光系统.
- 在广泛的光谱范围 (大约1100nm至2200nm) 中生成多个拉曼激光线.
- 脉冲能量,线宽 (<0.1 nm) 和与脂质,原和水吸收带重叠的特征光谱输出.
主要成果:
- 该ARHCF激光器成功地发射了多个光谱线,具有高脉冲能量和狭窄的线宽.
- 生成的线条与脂质 (1210 nm, 1700 nm),原 (~1540 nm) 和水 (~1400 nm, ~1900 nm) 的吸收峰值精确匹配.
- 使用开发的系统,在头发,猪组织和原样本中展示了有效的吸收物映射.
结论:
- 气填充ARHCF激光技术适用于先进的MS-OAM应用.
- 开发的源能够在扩展的光谱范围实现高分辨率,无标签的生物成像.
- 这项技术有可能将MS-OAM能力扩展到红外和紫外两种模式.
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