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Updated: Sep 11, 2025

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
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Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

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基于相位的时间域光寿命成像显微镜的量化不确定性

Qinyi Chen1, Jongchan Park1, Shuqi Mu1

  • 1Department of Bioengineering, University of California, Los Angeles, California 90095, USA.

Biomedical optics express
|August 14, 2025
PubMed
概括

这项研究引入了一种新的模型,用于理解基于相机的时间域光终身成像显微镜 (FLIM) 中的噪声. 该模型量化了光子射击噪声如何影响测量,提高了FLIM在低光条件下的可靠性.

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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

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Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

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相关概念视频

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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