相关实验视频

Updated: Sep 11, 2025

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

在Yarrowia lipolytica中通过CRISPR-Cpf1和正交T7系统进行多重基因组编辑和转录敲除

Hanqing Zhang^1,2, Luai R Khoury², Peng Xu^1,3

¹Department of Chemical Engineering, Guangdong Technion-Israel Institute of Technology, 515063 Shantou, China.

ACS synthetic biology

|August 14, 2025

概括

这项研究提出了一个新的CRISPR-Cpf1工具包,用于在Yarrowia lipolytica中高效的基因编辑和基因敲除. 该系统可实现多重编辑和转录控制,增强其在工业生物技术中的应用.

科学领域:

生物技术是生物技术.
合成生物学合成生物学
微生物工程微生物工程

背景情况:

雅罗维亚 (Yarrowia lipolytica) 是一种有价值的非传统酵母,用于生产脂质,油化学品和营养药品.
它的合成生物学工具包不如贝克酵母的工具包发达,限制了其工业应用.
有效的基因组工程和代谢途径控制对于优化Y. lipolytica作为工业宿主至关重要.

研究的目的:

为 Yarrowia lipolytica 开发和评估一个多重化的 CRISPR-AsCpf1 基因组编辑系统.
用CRISPR-dAsCpf1与正交T7促进器评估基因淘汰的效率.
为增强Y. lipolytica.的基因操纵提供一个简单的工具包.

主要方法:

利用CRISPR-AsCpf1系统与来自本地或T7促进者的指导RNA (gRNA) 来进行多重基因组编辑.
研究了提高同质导向重组 (HDR) 效率的策略.
与CRISPR-dAsCpf1介导的基因敲除与CRISPR-RfxCas13d和反意义T7促进器系统进行了比较.

主要成果:

使用CRISPR-AsCpf1.1.实现了最多4个目标基因的73.3%的编辑效率和2个基因的100%的编辑效率.
观察到对同质性导向重组效率的微小改进.
通过CRISPR-dAsCpf1和T7驱动的gRNA证明了显著的基因淘汰,与反意义系统相似.

结论:

开发的CRISPR-Cpf1工具包使Y. lipolytica. 的高效和多重基因组编辑成为可能.
该系统有助于有效的转录敲击目标基因.
这项工作为推进Y. lipolytica在工业生物技术应用中提供了有价值的工具.

关键词:

这就是CRISPR-AsCas12f1的特征.克里斯普尔-AsCpf1是什么意思克里斯普里是什么?克里斯普里是什么?它们是RNAiRNAi.T7转录系统的转录系统雅罗维亚 (Yarrowia lipolytica) 是一个高脂的植物.

更多相关视频

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Published on: February 28, 2025

相关概念视频

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...