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Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.

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Fluorescence detection methods for microfluidic droplet platforms
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同焦点吸收激活滴滴分类 (cAADS) 用于酶工程

Abdi Mirgissa Kaba1, Sébastien Gounel1, Thomas Beneyton1

  • 1Centre de Recherche Paul Pascal (CRPP), CNRS UMR 5031, Univ. Bordeaux, 115 Avenue du Docteur Schweitzer, Pessac, 33600, France.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|August 14, 2025
PubMed
概括
此摘要是机器生成的。

同焦点吸收激活滴滴分类 (cAADS) 允许使用吸收检测对酶变体进行敏感,高通量选. 这种微流体系统通过克服光基方法的局限性来加速酶工程.

关键词:
吸收活性激活的滴滴选方式bilirubin 氧化酶 的作用指导进化是指导进化的.基于滴滴的微流体制剂 微流体制剂高通量选的高通量选

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科学领域:

  • 生物技术是生物技术.
  • 化学工程是化学工程的重要组成部分.
  • 微流体学 微流体学

背景情况:

  • 定向进化需要对酶变异进行有效选.
  • 滴滴微流体提供高通量选,但受测试类型的限制.
  • 基于光的测试是常见的,但吸收性测试覆盖了更多的酶系统.

研究的目的:

  • 开发一种微流体系统,用于对酶变体进行敏感,高通量,基于吸收的选.
  • 为了克服现有的液滴微流体选方法的灵敏度和吞吐量限制.

主要方法:

  • 开发一个共聚焦吸收激活滴滴分类 (cAADS) 系统.
  • 使用皮科升尺度的油中的水乳液作为吸收度测量的微分区.
  • 使用胆红素氧化酶 (BOD) 和葡萄糖氧化酶变体进行演示.

主要成果:

  • 在超高吞吐量 (5.4 kHz) 实现了10 pL滴滴的敏感吸收度测量.
  • 能够在高达2.6 kHz的频率下对50 pL滴进行分类.
  • 对于活跃的BOD变体和Glucose Oxidase的多功能性来说,已经证明了99%的分类效率.

结论:

  • cAADS系统显著提高了酶工程的微流体选能力.
  • 这项技术扩大了滴滴微流体的适用性,用于基于吸收的测定.
  • cAADS为定向进化和酶发现提供了一个多功能和高效的平台.