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相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

220
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
220
CRISPR01:59

CRISPR

52.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.9K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.4K

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相关实验视频

Updated: Sep 11, 2025

CIRCLE-Seq for Interrogation of Off-Target Gene Editing
08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

858

通过两阶段深度学习框架预测CRISPR Cas9的目标外活动.

Tianshan Zhang, Bei Jiang, Guang Yang

    IEEE transactions on computational biology and bioinformatics
    |August 14, 2025
    PubMed
    概括

    我们开发了CAF-Net,这是一个深度学习框架,用于预测CRISPR-Cas9的目标外活动. 这种方法解决了指导RNA设计中的标签失衡问题,以提高基因组工程的准确性.

    科学领域:

    • 基因组学就是基因组学.
    • 分子生物学分子生物学
    • 生物信息学是一种生物信息学.

    背景情况:

    • CRISPR-Cas9系统是真核生物基因组工程的强大工具.
    • Cas9导向RNA的非目标活动构成了重大挑战,可能导致意外突变.
    • 目前用于预测非目标站点的现有方法由于众多潜在站点而与标签不平衡作斗争.

    研究的目的:

    • 开发一个准确的深度学习框架来预测CRISPR-Cas9的目标外活动.
    • 为了解决指导RNA设计的目标外预测中固有的标签失衡问题.

    主要方法:

    • 开发了CAF-Net (Cas9增强和Finetune网络),一个深度学习框架.
    • 预训练了一个嵌入模型,从目标中提取特征并引导RNA序列.
    • 应用数据增强到提取的特征和微调模型与合成样本.

    主要成果:

    • CAF-Net有效地预测了CRISPR-Cas9.9的目标外活动.
    • 该框架在已建立的数据集上展示了强大的性能.
    • 数据增强和微调策略缓解了标签不平衡问题.

    结论:

    更多相关视频

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    Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
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    Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

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    Last Updated: Sep 11, 2025

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    Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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    Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

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    Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
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    • CAF-Net提供了一种新且有效的解决方案,用于预测CRISPR-Cas9的目标外活动.
    • 开发的框架提高了引导RNA设计的准确性和可靠性.
    • 这种方法对安全和精确的基因组工程应用有重大影响.