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固体-液体提取到水友性深度环氧溶剂中,然后预缩到初级氨基基基上分子溶剂中:液体染色学-质谱测定肉中四环素的测定

Yaroslav Kurashov¹, Aleksei Pochivalov², Daria Frolova²

¹Department of Analytical Chemistry, Institute of Chemistry, Saint-Petersburg State University, Universitetskaya Emb. 7/9, 199034 St. Petersburg, Russia; Department of Analytical Chemistry, Certification and Quality Management, Kazan National Research Technological University, Karl Marx street 68, 420015 Kazan, Russia.

Journal of chromatography. A

|August 14, 2025

概括

研究人员开发了一种使用超分子溶剂预先缩极性分析物的新方法. 这种技术有效地在低检测极限的肉样本中确定四环素.

科学领域:

分析化学分析化学
超分子化学超分子化学

背景情况:

深度环氧化溶剂 (DES) 为分析物提取提供了独特的特性.
在水相中形成超分子溶剂是一种新奇的现象.
从复杂矩阵中预先缩极性分析物仍然具有挑战性.

研究的目的:

描述含有初级氨基和三成分DES的水相中的超分子溶剂形成.
为了实现这一现象用于极性分析物的预度.
开发一种测定肉样本中四环素的方法.

主要方法:

使用DES前体和一次性氨基的混合超分子聚合物的形成.
从肉中提取四环素的固体液体,使用胆化物-甘酸-水DES.
液体染色学-质谱学 (LC-MS) 用于测定四环素.
优化DES组成和两类选择 (1-nonylamine).

主要成果:

在水相中成功形成超分子溶剂.
有效的预度和分离四环素从肉矩阵.
优化的DES组成:胆化物:甘油酸 (1:1) 与20%的水.
通过LC-MS实现了使用四环素的低检测极限 (1-4μg kg-1).
在提取过程中证明了有效的蛋白质沉.

结论:

超分子溶剂形成为分析物预度提供了一种新的方法.
开发的方法是有效的,用于确定食品样本中的四环素.
这种技术为分析复杂矩阵中的极性化合物提供了一个有希望的替代方案.

关键词:

深度欧性溶剂溶剂在HPLC-MS/MS中使用.液相微提取液相微提取的方法固体-液体提取方法固体-液体提取方法这是一种超分子溶剂.环素的四环环素是一种.肉肉类肉肉类

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