肥胖和同热量控制饮食都会改变小鼠的微生物群组成,以性别依赖的方式
Ashley M Loeven1, Amber N Brown2, Francis U Ebuara1
1Department of Biological Science, The Florida State University, Tallahassee, FL 32306-4295, United States.
Physiology & behavior
|August 14, 2025
概括
过多的饮食脂肪会影响肠道微生物群的多样性和组成,即使不会导致肥胖. 这些变化在脂肪消费和过度消费之间存在差异,突出了对微生物群落的不同影响.
科学领域:
- 微生物学 微生物学
- 营养科学 营养科学
- 神经科学是一个神经科学.
背景情况:
- 嗅觉回路可以在过多的饮食脂肪摄入时失去结构和功能.
- 在没有明显肥胖的情况下,在同热量高脂肪饮食期间肠道微生物组的改变仍然未得到充分研究.
研究的目的:
- 为了研究肠道微生物组的变化,以适度高脂肪 (MHF) 饮食的反应使用同热量养.
- 为了比较5个月的同热量和任意高脂肪养之间的微生物变化.
主要方法:
- 在5个月内从被养的对照小鼠或MHF饮食 (异热量或自由选择) 收集便样本.
- 16S rRNA基因测序 (V4区域) 用于分析肠道微生物组的组成和多样性.
- 采用了阿尔法和β多样性指标,以及特定的细菌丰度分析.
主要成果:
- 在5个月后,MHF饮食在同热量和自由养的动物中同样减少了α多样性.
- 在接受MHF饮食的雌性小鼠中出现了独特的β多样性模式,但在雄性中却没有出现.
- 在接受MHF养的小鼠中观察到Firmicutes的增加和Bacteroidetes的减少;确定了特定的细菌协会 (Allobaculum,Muribaculaceae,Lactobacillus salivarius).
结论:
- 肠道微生物组调节在过度饮食脂肪消费和导致肥胖的过度消费之间存在差异.
- 与ad libitum过度消费相比,同热量MHF养会诱导明显的微生物转移.
- 微生物的动态,就像Ruminococcaceae的短暂变化一样,反映了饮食诱导的肥胖的早期阶段.
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