这是一种高效的电荷驱动方法,可以将菌体封装在脂质体中
Yue Cao1, Mengyu Li1, Dipesh Khanal1
1Advanced Drug Delivery Group, Sydney Pharmacy School, Faculty of Medicine and Health, The University of Sydney, NSW 2006, Australia.
International journal of pharmaceutics
|August 14, 2025
概括
一种新的静电方法有效地将菌体封装成脂质体,增强抗菌素治疗耐药感染的稳定性和传递性. 这种可扩展的方法实现了高效率,并保持了菌体的生存能力.
科学领域:
- 生物技术是生物技术.
- 抗菌疗法是一种抗菌疗法.
- 纳米技术纳米技术
背景情况:
- 脂质体封装细菌对通过改善菌体稳定性和向传递来治疗多抗药性感染充满希望.
- 现有的封装方法效率低 (≤50%),菌体不活化,工业生产的可扩展性差.
研究的目的:
- 开发和评估一种以静电驱动的封装方法,用于生产菌体脂肪体配方.
- 通过提高效率,可扩展性和菌体生存能力来克服当前方法的局限性.
主要方法:
- 使用了阴性脂质 (DOTAP) 和可扩展的微混合系统 (AXFTMmini,局限撞击喷气,微流体芯片).
- 研究了各种流速比率和总流速以优化封装.
- 采用传输电子显微镜,原子力显微镜和红外光谱仪进行结构分析.
主要成果:
- 通过静电相互作用,对于podovirus (PEV31) 和myovirus (PEV1) 实现了90-91%的高封装效率.
- 维持菌体活力与最小的减少 (<0.2 log10 位数).
- 产生的均大小的脂质体菌体 (<1000nm,PDI <0.3) 有轻微正的泽塔潜力.
结论:
- 静电封装方法有效地解决了菌体治疗的关键挑战,包括效率和可扩展性.
- 这种多功能策略可以容纳多种菌体形态,同时保持生存能力.
- 开发的脂质体菌体配方有可能用于工业规模的抗微生物应用.
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