相关实验视频

Updated: Sep 8, 2025

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Published on: June 13, 2011

通过SPON1-介导的ERK1/2/NF-κB通路的激活诱导神经炎症

Fangfei Li¹, Jie Zhang², Xiaoli Ma¹

¹Department of Toxicology, School of Public Health, Guangxi Medical University, Nanning, Guangxi 530021, China; Guangxi Key Laboratory of Environment and Health Research, Guangxi Medical University, Nanning, Guangxi 530021, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning, Guangxi 530021, China.

Neurotoxicology and teratology

|August 14, 2025

概括

(Mn) 暴露会降低SPON1水平,激活ERK1/2/NF-κB等炎症途径,并促进神经炎症. 恢复SPON1可能会减轻Mn诱导的认知障碍.

科学领域:

神经科学是一个神经科学.
毒理学毒理学毒理学
分子生物学分子生物学

背景情况:

过度的 (Mn) 积累会导致神经炎症和认知缺陷.
细胞外基质糖蛋白SPON1与神经炎症有关,但其在Mn诱导炎症中的作用尚不清楚.

研究的目的:

为了研究SPON1在诱导的神经炎症中的作用.
阐明Mn影响SPON1和炎症通路的机制.

主要方法:

在体内研究:雄性C57BL/6小鼠在12周内接受了Mn (0-100 mg/kg) 的挖掘.
在体外研究:初级神经元,N2a和BV2细胞暴露于Mn.
测量了SPON1表达和ERK1/2/NF-κB通路的激活.

主要成果:

在小鼠皮质和体外细胞模型中,Mn暴露降低了SPON1的表达.
在小鼠皮层中,Mn暴露对ERK1/2和NF-κB通路蛋白进行了上调.
SPON1倒置加剧了炎症;SPON1过度表达减少了炎症和通路激活.

结论:

接触会抑制SPON1,导致ERK1/2/NF-κB通路激活和神经炎症.
在Mn诱导的神经毒性中,SPON1可能是关键的调解者.
针对SPON1可能是治疗Mn相关认知功能障碍的治疗策略.

关键词:

在ERK1/2中.这就是为什么Mn Mn Mn Mn 在 NF-κBB 中.神经炎症是一种神经炎症.这就是SPON1的意义.

更多相关视频

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

Published on: July 12, 2012

In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice

In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice

Published on: July 22, 2014

相关概念视频

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...

MAPK Signaling Cascades

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...