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集团SPL促进了STING和IRF3介导的抗病毒免疫反应,以对抗虹膜病毒感染

Kaitao Xi¹, Yunyong Jin¹, Long Lin¹

¹College of Marine Sciences, South China Agricultural University, Guangzhou, China.

Fish & shellfish immunology

|August 14, 2025

概括

群英雄素-1-酸酶 (EcSPL) 作为一种抗病毒因子,限制新加坡群英雄伊利多病毒 (SGIV) 复制. 通过促进对SGIV感染的STING和IRF3介导反应,EcSPL增强了宿主天生的免疫力.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
生物化学生物化学

背景情况:

氨酸-1-酸盐 (S1P) 解酶 (SPL) 是一种细胞内酶,可降解生物活性脂质S1P.
新加坡虹膜病毒 (SGIV) 在水产养殖业造成重大经济损失.
SPL在鱼类抗病毒免疫力中的作用尚不清楚.

研究的目的:

调查SPL (EcSPL) 在对SGIV感染的先天免疫反应中的作用.
阐明EcSPL影响抗病毒免疫力的分子机制.

主要方法:

定量实时PCR (qPCR) 用于基因表达分析.
亚细胞局部化研究.
过度表达测试以评估抗病毒效应和信号通路激活.
共同免疫沉以确定蛋白质相互作用.

主要成果:

埃克斯普尔表达是由SGIV感染诱导的,并在细胞质,ER,线粒体和溶解体中局部化.
EcSPL过度表达显著抑制了SGIV复制,病毒基因表达和细胞病变效应.
埃克斯普尔增强了干扰素1 (IFN1),干扰素3 (IFN3) 和核因子kappa-B (NF-κB) 的促进活性,特别是当由STING和IRF3.3触发时.
埃克斯普尔直接与STING和IRF3.3互动并与之共定位.

结论:

EcSPL作为宿主对SGIV感染的天生的免疫力的积极调节者.
EcSPL通过增强STING和IRF3介导的抗病毒免疫反应来限制SGIV复制.
这项研究揭示了EcSPL作为远鱼的抗病毒宿主因子的新作用.

关键词:

集团集团是一个集团.在 IRF3 中,IRF3 是 IRF3 的一个类型.免疫反应是免疫反应.美国国务院GVIV 在SPL中,SPL是SPL.刺痛是一种刺痛.

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