通过LC-MS/MS对人体血和骨组织中的素简单且可靠的分析
Qixian Ling1,2, Yuanyuan Zhang1,2, Haotian Ma1,2
1Department of Pharmacy, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100191, China. xiongxin1029@outlook.com.
Analytical methods : advancing methods and applications
|August 14, 2025
概括
一种新方法准确地测量了血和骨中的素含量,这对于预防关节置换手术后的感染至关重要. 这支持优化抗生素的使用,以减少假肢关节感染风险.
科学领域:
- 药物动力学和制药分析
- 整形外科手术和传染病.
背景情况:
- 全关节整形术 (TJA) 对末期骨关节炎来说是绝对的,但假肢关节感染 (PJIs) 是一个越来越令人担忧的问题,特别是随着修复手术的增加.
- 素对手术抗微生物预防 (SAP) 针对格拉姆阳性和格拉姆阴性细菌至关重要,但其有效性取决于在骨植入物接口达到适当度.
- 优化赛福洛的位药理动力学对于减轻TJA患者的PJI风险至关重要.
研究的目的:
- 开发和验证一种灵敏的液体染色学-并联质谱法 (LC-MS/MS) 试验.
- 为了在人体血和骨组织样本中同时量化素.
- 为了支持药理动力学研究,以优化全关节关节整形术中的 cefuroxime 预防.
主要方法:
- 一种经过验证的LC-MS/MS方法被用来量化Cefuroxime.
- 使用BEH C18柱实现了分离,在乙尼和水中对酸进行梯度化.
- 测试性能被评估为准确性,精度,矩阵效应,提取回收和在各种条件下的稳定性.
主要成果:
- 在LC-MS/MS试验中,校准曲线的相关系数 (>0.99) 高.
- 精度在血中为93.11%至98.60%,在骨中为89.15%至106.2%.
- 测试内部和测试间的精度处于可接受的范围内 (≤15%或20%在LLOQ),矩阵效应最小,提取回收效率高.
结论:
- 开发的LC-MS/MS方法是稳健的,准确的,准确的量化人体血和骨组织中塞福洛キシ姆.
- 该方法在各种条件下的稳定性确保了可靠的样本分析.
- 这种经过验证的测定方法适用于确定临床样本中的 cefuroxime 度,有助于优化抗菌预防PJIs.
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