一种广泛中和的抗体识别出一种独特的表位,具有冠状病毒中普遍存在的特征图案
Lei Yan1, Fulian Wang1, Michelle Hill2
1Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China.
Nature communications
|August 14, 2025
概括
一种新型抗体,3D1,向一种保存的病毒毛前结构,中和了许多Sarbecoviruses. 这种广泛中和抗体 (bnAb) 揭示了各种RNA病毒中存在的交叉反应性表位.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 结构生物学 结构生物学
背景情况:
- 在Sarbecoviruses中已知交叉反应性抗体,但它们的分子机制尚不清楚.
- 识别保存的病毒点对于广泛的抗病毒疗法至关重要.
研究的目的:
- 为了隔离和表征一种广泛中和抗体 (bnAb),针对Sarbecoviruses中的保存表位.
- 阐明抗体介导交叉反应的分子机制.
主要方法:
- 从人类组合抗体库中分离bnAb (3D1).
- 由3D1识别的表位的特征,包括其结构特征和病毒背景.
- 评估3D1对各种SARS-CoV-2菌株和其他RNA病毒的中和活性.
主要成果:
- 3D1的目标是HR1域中保存的毛前过渡状态,特别是β转折折.
- 这种表位在冠状病毒和其他RNA病毒 (如HIV和马尔堡病毒) 中存在.
- 3D1中和了大多数SARS-CoV-2野生型菌株,但由于Q954H突变,Omicron可以逃避它.
结论:
- 由3D1识别的神秘表位代表了各种RNA病毒的保护漏洞.
- 抗体库方法对于发现bnAbs向保存的病毒结构是有效的.
- 3D1是一个能够广泛结合抗原的天然抗体的例子,突出显示了潜在的治疗策略.
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