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通过LAP-MALDI MS对儿童牙的分析,快速而准确的生物性别估计
Lily R Adair1, Mary E Lewis2, Rainer Cramer1
1Department of Chemistry, University of Reading, Whiteknights, Reading, RG6 6DX, UK. r.k.cramer@reading.ac.uk.
The Analyst
|August 15, 2025
概括
这项研究验证了一种使用牙面膜的生物性别估计快速方法. LAP-MALDI MS工作流在成年人中实现了100%的准确性,并在儿童中显示出有希望的结果.
科学领域:
- 法医人类学 法医人类学
- 生物化学 生物化学
- 考古学的考古学
背景情况:
- 从骨遗骸中估计生物性别在考古学和法医学中至关重要.
- 传统的骨科可能具有挑战性,特别是碎片化或不成熟的骨材料.
- 此前已经引入了一种使用LAP-MALDI MS和MS/MS分析原蛋白的新型快速工作流.
研究的目的:
- 在中世纪人类牙上应用和验证生物性别估计的新快速工作流.
- 将LAP-MALDI MS方法的结果与传统的骨科评估进行比较.
- 评估工作流程对成人和儿童骨遗骸的有效性,包括落叶牙.
主要方法:
- 使用激光切除-MALDI质谱法 (LAP-MALDI MS) 和MS/MS.分析牙面膜中的阿梅洛金.
- 开发和应用基于MS和MS/MS离子信号的分类规则.
- 将LAP-MALDI MS结果与传统的骨科性别确定方法进行比较.
主要成果:
- 在LAP-MALDI MS和骨科之间100%的协议对五个成年人的生物性别估计.
- 成功估计了四个儿童个体的生物学性别,其中包括三个落叶牙.
- 在两个儿童病例中观察到差异,其中LAP-MALDI MS提供了更清晰的性别识别,包括Y染色体检测.
结论:
- 快速的LAP-MALDI MS工作流程是一种可靠和准确的方法,用于从人类牙面膜中估计生物性别.
- 这种方法与成年人的骨科学具有很高的一致性,并为未成熟的个人提供了宝贵的见解.
- 工作流的检测特定的能力,如Y染色体SMoxIRPPY,增强了其诊断能力.
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