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器官内内皮细胞的空间时间增殖异质性

Maoying Han^1,2,3, Zhicong Liu⁴, Lei Liu²

¹CAS CEMCS-CUHK Joint Laboratories, New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai (M.H., X.H., B.Z.).

Circulation research

|August 15, 2025

概括

内皮细胞 (EC) 增殖在心脏,肝脏和肺部等器官内有显著的变化. 这项研究揭示了独特的EC亚型和区域驱动这种异质性,这对血管再生至关重要.

科学领域:

血管生物学血管生物学
再生医学是一种再生医学.
细胞异质性细胞异质性

背景情况:

血管的完整性需要持续的内皮细胞 (EC) 复制.
ECs显示已知的组织特异性,但器官内增殖差异未得到充分研究.

研究的目的:

调查心脏,肝脏和肺部内ECs的增殖异质性.
发现导致EC扩散差异的分子机制.
评估MAPK信号在EC异质性中的作用.

主要方法:

开发了一个EC专用的扩散追踪系统.
采用RNA测序,空间转录组学和单细胞RNA测序.
在体内使用MAPK抑制和器官损伤模型.

主要成果:

在心脏,肝脏和肺部发现明显的器官内EC增殖异质性.
确定了具有明显的繁殖能力的特定EC亚型 (例如,E-CAD±1,PLVAP+,CAR4+).
多组学揭示了区域转录的多样性,并证实了MAPK通路的作用.

结论:

由独特的基因表达程序驱动的未发现的区域和亚型特定的EC扩散.
突出了微血管EC的空间和功能多样性.
为制定器官特异性血管再生策略提供了一个框架.

关键词:

细胞因子细胞因子它们是内皮细胞.恒常状态 (homeostasis) 是一种平衡状态.激素激素激素激素激素肝脏肝脏肝脏肝脏肝脏肝脏

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