相关实验视频

Updated: Sep 11, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

克里斯普尔编辑细胞系:功能瘤学研究的新时代

Amita Joshi Rana¹, Md Sadique Hussain², Ali Hanbashi³

¹Graphic Era Hill University College of Pharmacy Bhimtal India.

Current pharmaceutical design

|August 15, 2025

概括

克里斯普尔-Cas9基因编辑通过使精确的药物反应研究和改善免疫疗法来增强癌症研究. 这项技术有助于跟踪癌症的演变,并制定个性化的治疗策略.

科学领域:

基因组学就是基因组学.
癌症生物学癌症生物学
免疫治疗是一种免疫疗法.

背景情况:

克里斯普尔-Cas9技术允许精确的工程癌症细胞系.
了解癌细胞对疗法的反应对于有效治疗至关重要.

研究的目的:

突出CRISPR-Cas9在癌症研究中的进展和应用.
探索其在药物耐药性研究,免疫疗法和追踪癌症演变中的作用.

主要方法:

使用CRISPR-Cas9进行基因淘汰 (例如,MED12) 和建模癌症过程 (例如,上皮层-介质细胞转换).
应用CRISPR来增强化学抗原受体T (CAR-T) 细胞疗法,通过破坏免疫检查点 (PD-1,CTLA-4).
在多种癌症细胞系中进行药物基因组分析.

主要成果:

能够详细检查癌细胞对药物反应和抗药机制.
促进了针对特定抗原的改进CAR-T细胞疗法的开发.
允许追踪癌症演变,表观遗传变化以及在NSCLC和卵巢癌中的抵抗逆转策略.

结论:

克里斯普尔-Cas9是一种强大的工具,可以彻底改变癌症研究和治疗方法的开发.
未来与人工智能,多组学和有机体的整合将促进精确瘤学和治疗规划.

关键词:

这就是CRISPR/Cas9的作用.癌症细胞系癌症细胞系药物耐药性耐药性药物耐药性基因编辑基因编辑精确瘤学精确瘤学瘤的进展.

更多相关视频

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Published on: January 3, 2015

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Published on: November 8, 2017

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...