使用紫外光学声波弹性成像和光学连贯显微镜,对未经加工的生物组织进行多式无标签成像
Optics letters
|August 15, 2025
概括
这项研究引入了一种新的无标签成像技术,即紫外线光声波弹性成像 (UV-PAE) 和光学连贯显微镜 (OCM),用于快速组织分析. 这种方法通过减少对组织病理学进行漫长的样本准备来显著加快疾病诊断的速度.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 组织病理学 组织病理学
- 摄影声学 摄影声学
- 光学连贯性断层扫描技术
背景情况:
- 组织组织学组织分析对于疾病诊断至关重要,但传统方法耗时.
- 当前的组织病理学技术需要大量的样本准备,导致诊断延迟.
研究的目的:
- 开发一种无标签的多式显微镜技术,用于快速组织学可视化.
- 为了减少与传统组织组织病理学准备相关的时间和劳动力.
主要方法:
- 开发了一种多模式显微镜系统,结合了反射模式紫外线光声学弹性学 (UV-PAE) 和近红外光学连贯显微镜 (NIR-OCM).
- 利用机械弹性和光学散射对比度进行无标签成像.
- 将该技术应用于小鼠大脑和乳腺瘤组织.
主要成果:
- 在无需大量准备的情况下,UV-PAE/NIR-OCM方法揭示了小鼠大脑组织的多参数结构和形态细节.
- 该技术成功地在小鼠乳腺瘤标本中确定了病理特征.
- 新方法的结果与传统的组织学染色图像一致.
结论:
- 拟议的UV-PAE/NIR-OCM是无标签组织学成像技术的进步.
- 这种技术提供了一个快速有效的方法,用于手术内组织病理学与最小的样本准备.
- 它有可能减少临床环境中的诊断延迟.
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