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鉴定bla_ESBL-和bla_CARBA-积极的多药耐药混合物calida从不同的人类宿主中分离出来的单独物种

Francesca McDonagh¹, Kate Ryan¹, Aneta Kovářová¹

¹Antimicrobial Resistance and Microbial Ecology Group, School of Medicine, University of Galway, Galway, Ireland.

International journal of medical microbiology : IJMM

|August 15, 2025

概括

在人体样本中发现了两种携带碳烯酶基因的多抗药性Mixta calida分离物. 基因组分析表明单一的引入事件,突出显示了横向基因转移在传播耐药性的作用以及加强监测的必要性.

科学领域:

医学微生物学医学微生物学
基因组学就是基因组学.
抗微生物耐药性抗微生物耐药性

背景情况:

多药耐药 (MDR) 细菌的出现构成了严重的公共卫生威胁.
米克斯塔·卡利达是一种新兴的病原体,对其耐药性机制的数据有限.
碳酶基因 (blaCARBA) 对细菌对最后手段抗生素的耐药性至关重要.

研究的目的:

从人类宿主中识别和描述blaCARBA阳性MDR Mixta calida分离物.
阐明M. calida. 多药耐药性的基因组决定因素.
了解这些耐药分离物种的全物种背景.

主要方法:

MALDI-ToF质谱和16SrRNA测序用于隔离物识别.
全基因组测序以识别耐药性基因和等离子体复制物.
按照EUCAST和CLSI的指导方针进行抗菌敏感性测试.
与现有的M. calida基因组进行比较基因组分析.

主要成果:

从爱尔兰不同的人类来源鉴定出两种MDR M. calida分离物.
分离物中含有由等离子体传播的blaKPC-3,blaOXA-9和blaTEM-122基因.
遗传学分析表明,这些耐药菌株的单一引入事件.
在分离物之间观察到共享的等离子体复制体类型.

结论:

这项研究报告了在人类殖民和感染中首次出现blaCARBA阳性M. calida的双重发生.
横向基因转移是MDR M. calida.出现的一个关键驱动因素.
加强监测,诊断和感染控制对于减轻公共卫生风险至关重要.

关键词:

一个叫做 Mixta Calida 的小组.bla ((KPC-3)) 的使用情况.bla(OXA-9) 的使用情况.bla{(TEM-122) 的一个例子.

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