相关实验视频

Updated: Sep 11, 2025

Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions

Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions

Published on: April 19, 2013

最近在了解P型IIIAATPase介导的细胞pH值和植物适应环境变化的离子稳态方面取得的进展

Qian Li¹, Xin Jiang¹, Yi Liu¹

¹Fujian Provincial Key Laboratory of Plant Functional Biology, College of Life Sciences, Fujian Agriculture and Forestry University, 350002, Fuzhou, PR China.

Plant physiology and biochemistry : PPB

|August 15, 2025

概括

等离子膜质子 (H⁺-ATPases) 对植物生长和环境反应至关重要. 本综述详细介绍了它们在pH和离子稳态中的功能,这对植物细胞功能至关重要.

科学领域:

植物生理学植物生理学
分子生物学分子生物学
生物化学生物化学

背景情况:

血 (PM) H+-ATPases作为质子起作用,对植物生长和发育至关重要.
它们产生跨膜pH梯度 (ΔpH) 和电化学潜力 (ΔΨ),影响细胞过程.
质子介导的pH缓冲和离子稳态的最新决定因素仍在讨论中.

研究的目的:

将P型ATPases分类,并通过PM H+-ATPases审查H+运输机制.
探索PM H+-ATPases在控制细胞质pH和离子恒温中的作用.
为了检查PM H+-ATPases在正常和压力条件下如何与其他细胞组件相互作用.

主要方法:

文献综述和P型ATPases的分类.
在植物等离子体膜中分析H+运输机制.
整合有关PM H+-ATPase,离子通道,抗载体和代谢过程的数据.

主要成果:

PM H+-ATPases是植物细胞电化学渐变的关键调节者.
这些,与其他输送器一起,维持细胞质pH和离子平衡.
了解这些机制对于植物适应各种条件至关重要.

结论:

PM H+-ATPases在植物离子和pH平衡中发挥着核心作用.
它们与其他细胞因子的相互作用决定了植物对环境刺激的反应.
对这些相互作用的进一步研究将推动植物科学和耐压力策略的发展.

关键词:

阴离子/质子反载波器.循环核酸带通道的通道.代谢过程中的代谢.P型的ATP酶是一种P型的ATP酶.血中的H(+) -ATPase酶

更多相关视频

Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis

Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis

Published on: June 4, 2021

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy

Published on: April 28, 2014

相关概念视频

ATP Driven Pumps I: An Overview

ATP Driven Pumps I: An Overview

ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...

ATP Driven Pumps III: V-type Pumps

ATP Driven Pumps III: V-type Pumps

V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...

ATP Driven Pumps II: P-type Pumps

ATP Driven Pumps II: P-type Pumps

The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...

pH Regulation in Cells

pH Regulation in Cells

pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...

Regulation of Transpiration by Stomata

Regulation of Transpiration by Stomata

During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.

Tonicity in Plants

Tonicity in Plants

Plant cells maintain appropriate osmotic balance in extreme conditions. For instance, plants in dry environments store water in vacuoles, limit the opening of their stoma, and have thick, waxy cuticles to prevent unnecessary water loss. Some species of plants that live in salty environments store salt in their roots. As a result, water osmosis occurs in the root from the surrounding soil.
Tonicity
Tonicity describes the capacity of a cell to lose or gain water depending on the solute...