与颗粒相关的和自由存在的Legionella spp. 在饮用水中
Danielle M Angert1, Katherine S Dowdell2, Lan Nguyen1
1The University of Texas at Austin, Department of Civil, Architectural, and Environmental Engineering, 301 E. Dean Keeton St., Stop C1786, Austin, TX, 78712, USA.
Water research
|August 15, 2025
概括
在饮用水中,自由活的莱吉欧内拉菌比与粒子相关的细菌更为常见. 这一发现对于了解暴露风险和制定有效的水处理策略来预防军人病至关重要.
科学领域:
- 环境微生物学环境微生物学
- 水中传播的病原体
- 公共卫生 公共卫生
背景情况:
- 军团菌种是机会性病原体,会引起军团病,包括军团病.
- 菌 (Legionella pneumophila,Lp) 在饮用水分配系统 (DWDS) 中持续存在,原因是生物膜/虫的抵抗和保护.
- 了解军团菌与粒子的关联与自由生活状态的关联对于有效的补救至关重要.
研究的目的:
- 量化饮用水中的自由活体与与粒子相关的莱吉欧内拉菌种和Lp.
- 调查水源类型和采样地点对莱吉欧内拉存在和多样性的影响.
主要方法:
- 公民科学家在三个季节内在美国收集了首次抽取和冲洗的饮用水样本.
- 微生物被用膜过方式以大小分成部分.
- 使用数字PCR进行了军团菌量化,并通过片测序评估了多样性.
主要成果:
- 在自由生活的Legionella spp. 它们的度显著高于与颗粒相关的Legionella spp.相比. (p=2.86×10−9) 的意思.
- 表面水系统的总体Legionella spp.显著更高. 度比地下水系统高 (p=0.020).
- 雷吉欧内拉肺炎的检测是零星的;地点强烈影响了雷吉欧内拉物种多样性.
结论:
- 源水类型和地点是影响Legionella spp.的重要因素. 在DWDS和管道.
- 消费者更有可能接触到自由生活的Legionella spp. 而不是自来水中的颗粒相关形式.
- 调查结果为管理饮用水系统中军团菌风险的策略提供了信息.
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