道鱼TCRβ和IGH受体谱 - 公共克隆类型补充了一个非常多样化的谱
R Craig Findly1, Ryan P Sweeney1, Frank D Niagro1
1Department of Infectious Diseases, College of Veterinary Medicine, The University of Georgia, Athens, GA, 30602, United States.
Developmental and comparative immunology
|August 15, 2025
概括
道鱼的T和B细胞受体表现出了显著的序列多样性. 一小组公共克隆类型,潜在的生殖系编码,在TCRβ和IGH受体中与高度多样化,独特的克隆类型共存.
科学领域:
- 免疫学 免疫学 免疫学
- 水生动物卫生 水生动物卫生
- 基因组学就是基因组学.
背景情况:
- 适应性免疫依赖于不同的T细胞受体 (TCR) 和B细胞受体 (BCR) 谱.
- 了解鱼类的免疫受体多样性对于水产养殖和疾病耐药性至关重要.
研究的目的:
- 为了比较T细胞受体β (TCRβ) 和免疫球蛋白重链 (IGH) 在道鱼中的序列多样性.
- 调查寄生虫感染和疫苗接种对免疫受体谱的组成的影响.
主要方法:
- 在28个月的时间里,从道鱼 (Ictalurus punctatus) 的cDNA库中进行高通量测序.
- 在寄生虫感染或接种疫苗之前和之后从血液,,皮肤和头部中采集的样本.
- 对TCRβ和IGH受体的互补性确定区域3 (CDR3) 序列的分析.
主要成果:
- 无论是TCRβ还是IGH的曲目,都呈现出高比例的曲目 (大约. 70%) 的独特克隆类型只检测一次.
- 在鱼类和时间点中,有一小部分公众克隆类型 (2 TCRβ,8 IGH) 始终被检测到.
- 公共克隆类型表明存在生殖系编码的VDJ基因重排.
- 缺乏V和D基因段的无菌TCRβ序列是丰富的.
结论:
- 道鱼免疫受体谱包括一组多样化的独特克隆类型和一组较小的公共克隆类型.
- 公众克隆类型的存在表明了潜在的生殖系对谱系的贡献.
- 这种双重谱结构可能是脊椎动物中保存的特征.
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