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相关概念视频

Protein Organization01:24

Protein Organization

7.0K
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence....
7.0K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.6K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.6K
Protein Complex Assembly02:41

Protein Complex Assembly

10.9K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.9K
Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

21.4K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
21.4K
Protein Families02:47

Protein Families

15.7K
Protein families are groups of homologous proteins; that is, they have similarities in amino acid sequences and three-dimensional structures. Protein families usually occur because of gene duplication, where an additional copy of a gene is inserted into the genome of an organism.   Mutations that change the amino acids but still allow the protein to be properly synthesized, will lead to new protein family members.   If these new proteins contain similar amino acids in key...
15.7K
Protein and Protein Structure02:15

Protein and Protein Structure

81.3K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
81.3K

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相关实验视频

Updated: Sep 11, 2025

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode
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NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode

Published on: June 4, 2021

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使用蛋白质块从蛋白质结构构建自定义碎片库.

Surbhi Dhingra1, Stéphane Téletchéa2, Ramanathan Sowdhamini3

  • 1Nantes Université, CNRS, US2B, UMR 6286, F-44000, Nantes, France; Computational Approaches to Protein Science (CAPS), National Centre for Biological Sciences (NCBS), Tata Institute for Fundamental Research (TIFR), Bangalore, 560-065, India.

Biochimie
|August 15, 2025
PubMed
概括

本研究介绍了一种使用蛋白质块 (PB) 来创建定制蛋白质片段库的新方法. 这种方法有效地发现了多样化,高质量的结构碎片,甚至从蛋白质中发现了无序区域.

关键词:
基于碎片的设计设计.当地脊柱形状的局部形状.蛋白质块可以阻断蛋白质.蛋白质结构预测 蛋白质结构预测结构字母表结构字母表

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科学领域:

  • 结构生物学是结构生物学.
  • 生物信息学是一种生物信息学.
  • 计算生物学是一种计算生物学.

背景情况:

  • 由于进化,蛋白质结构表现出了显著的多样性,但在同类和无关的蛋白质中保留了结构特征和图案.
  • 超次级动图是丰富而坚固的结构单元,突出显示了蛋白质中保存的局部脊柱形状.

研究的目的:

  • 介绍一种用于使用蛋白质块 (PBs) 创建定制蛋白质片段库的新管道.
  • 为了能够有效地提取和识别结构相似的蛋白质碎片,独立于序列同质.

主要方法:

  • 开发了一种管道,将3D蛋白质结构转换为1D蛋白质块 (PB) 序列,这是局部骨干构造的结构字母表.
  • 集成预测PB序列使用PB-ALIGN和PB-kPRED工具,用于同质独立的片段识别.
  • 实施了一个新的评分功能,将二次结构相似性和PB对齐指标结合起来,以评估片段质量.

主要成果:

  • 该管道有效地从一个精心策划的,非冗余的蛋白质结构数据库中提取结构相似的碎片.
  • 生成的库包含至少7个PB (11个氨基酸残留物) 的碎片,覆盖超过70%的本地骨干结构.
  • 成功地从各种蛋白质空间中提取了高质量的结构碎片,包括蛋白质乱的区域.

结论:

  • 蛋白质块 (PBs) 提供了一种有效的手段,可以在各种蛋白质结构中挖掘高质量的结构碎片.
  • 开发的管道 (PB-Frag) 提供了一个可访问的在线工具,用于生成定制的蛋白质片段库.