相关实验视频
Updated: Sep 11, 2025

07:05
Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
Published on: September 8, 2021
2.5K
在小鼠组织中转录延长焦点的组织和动态
Chihiro Matsuda1, Akane Ichiki2, Yuko Sato3
1School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan; School of Life Science and Technology, Institute of Science Tokyo, Yokohama 226-8501, Japan.
Journal of molecular biology
|August 15, 2025
概括
新的实验小鼠可视化了RNA聚合酶II Ser2酸化 (Ser2ph) 焦点,揭示了细胞特异的转录动态. 该工具绘制组织中活跃的基因转录部位的地图,帮助研究基因调节和细胞分化.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- RNA聚合酶II (RNAP2) 对于转录大多数真核生物基因至关重要.
- 在Ser2 (Ser2ph) 中RNAP2的酸化标志着生产性的转录延长.
- 现有的Ser2ph抗体允许在固定的样本中进行可视化.
研究的目的:
- 为了生成和描述表达光标记细胞内抗体 (薄荷体) 对RNAP2 Ser2ph.表达的 knock-in小鼠.
- 为了可视化和分析活鼠组织中的转录延长焦点.
- 研究这些焦点的分布,动态和细胞类型特定变异.
主要方法:
- 产生无处不在表达RNAP2 Ser2ph特异性薄荷体的敲入小鼠,与光蛋白融合.
- 在各种小鼠组织中可视化转录延长焦点,使用mintbody.
- 对焦点分布,数量,流动性和从异色色素中排除的定量分析.
主要成果:
- 在核区域成功可视化了数百到数千个RNAP2Ser2ph-mintbody焦点,不包括异色素和压抑的域.
- 观察到显著的组织和细胞类型特异性变异在转录焦点的数量和移动性.
- 与繁殖细胞相比,在分化的细胞中,焦点的运动性有所降低,与染色质可访问性相关.
结论:
- RNAP2 Ser2ph-mintbody敲进小鼠是研究体内转录延长动态的宝贵工具.
- 转录延长的空间组织和动态与细胞身份和分化状态有关.
- 研究结果提供了对不同细胞类型和发育阶段的基因表达调节的见解.
相关概念视频
Transcription Elongation Factors
11.1K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
11.1K
General Transcription Factors
5.5K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.5K
Transcription Initiation
16.8K
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
The promoters and enhancers and their accessory proteins allow tight regulation of...
16.8K
Transcription Factors
76.8K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
76.8K
Transcription in Prokaryotes
252
Transcription is a highly regulated process that converts genetic information into RNA molecules. The transcription cycle is divided into three key stages: initiation, elongation, and termination, each driven by specific molecular mechanisms.Initiation of TranscriptionIn bacteria, transcription begins when the RNA polymerase core enzyme associates with a sigma factor to form a holoenzyme. For example, the E. coli sigma factor called σ70 forms a holoenzyme, which recognizes the -10 (Pribnow...
252
Chromatin Position Affects Gene Expression
23.7K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
23.7K

