在anammox反应堆中去除和N2O排放:反应堆设计对过程性能和微生物群落的影响
Jean De Dieu Shema1, Satoshi Nakai1, Takehiko Gotoh1
1Graduate School of Advanced Science and Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima, 739-8527 Hiroshima, Japan.
Journal of bioscience and bioengineering
|August 15, 2025
概括
这项研究表明,测序批量反应堆 (SBR) 在废水处理过程中在去除和减少氧化 (N2O) 排放方面优于连续水箱反应堆 (CSTR). 较高的anammox生物量改善了SBR中的性能和降低了N2O输出.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 化学工程是化学工程的重要组成部分.
背景情况:
- 来自anammox工艺的氧化 (N2O) 排放给可持续的废水处理带来了挑战.
- 优化反应堆设计对于提高去除效率 (NRE) 和减轻温室气体排放至关重要.
研究的目的:
- 评估和比较不同安纳莫克斯反应堆配置中的去除效率 (NRE),N2O排放和微生物社区动态.
- 确定影响废水处理中NRE和N2O生产的关键微生物和遗传因素.
主要方法:
- 三个实验室规模的阿纳莫克斯反应堆的比较:SBR与循环复制,CSTR没有循环复制 (CSTR1) 和CSTR与循环复制 (CSTR2).
- 两个操作阶段具有不同的anammox生物质度 (1.63 g L-1和5.44 g L-1).
- 对去除,N2O排放,微生物多样性 (香农) 和基因丰度 (16S rRNA,nirS,nirK,nosZ) 的分析.
主要成果:
- 与两种CSTR配置相比,SBR显示出优异的NRE和显著较低的N2O排放.
- 增加anammox生物量增加了NRE和减少了N2O排放,特别是在SBR.
- 微生物多样性通常随着操作而下降,CSTR1的多样性更高与较低的anammox丰度和性能相关.
- 候选菌Jettenia caeni主导着anammox种群;更高的anammox 16S rRNA基因丰富与更好的NRE相关,而nirS和nirK基因丰富显示了反向关系.
结论:
- 序列批量反应器 (SBR) 在基于anammox的废水处理中,在同时去除和控制N2O排放方面比CSTR更有效.
- 反应堆的配置和运行参数显著影响微生物群体结构,去除和N2O缓解.
- 亚纳莫克斯细菌的丰富性和特定的功能基因 (nirS,nirK,nosZ) 在调节处理性能和温室气体排放方面发挥着至关重要的作用.
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