Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.2K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.2K
Differential Staining Technique01:26

Differential Staining Technique

615
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
615
Methods of Classification and Identification01:28

Methods of Classification and Identification

187
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
187
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

141
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
141

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Whole genome sequence analysis of eight <i>Bacillus</i> isolates from sugar beet factory juice and biofilms.

Microbiology resource announcements·2026
Same author

Optimizing antimicrobial dosing of bacterial isolates from sugar beet factories to reduce sucrose losses.

Applied and environmental microbiology·2026
Same author

Can antimicrobial peptides provide an improved antimicrobial strategy for treatment of <i>Cutibacterium acnes</i> prosthetic joint infection?

Microbiology spectrum·2025
Same author

Whole genome sequencing of <i>Leuconostoc suionicum</i>, <i>L. mesenteroides</i>, and <i>L. citreum</i> isolates from sugar beet factory juice and biofilms.

Microbiology resource announcements·2025
Same author

Genomic insights into persistence, antibiotic resistance, and intraspecific diversity of lactic acid bacterial contaminants at corn dry-grind fuel ethanol facilities.

Bioresource technology·2025
Same author

Fungal and Bacterial Species Associated with Storage Diseases in Sugar Beet from the Red River Valley of Minnesota and North Dakota.

Plant disease·2025

相关实验视频

Updated: Sep 11, 2025

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

11.6K

通过FTIR光谱学,机器学习和基因组学,从糖作物加工的微生物分离物中分类德克斯和莱万.

Evan Terrell1, Gillian O Bruni1, Yunci Qi1,2

  • 1US Department of Agriculture, Agricultural Research Service, New Orleans, LA, USA.

Journal of the science of food and agriculture
|August 15, 2025
PubMed
概括

微生物衍生的外聚糖 (EPS),如德克斯和莱文果糖,在糖制造中造成加工问题. 这项研究介绍了一种低成本的富里埃变换红外光谱法 (FTIR) 方法,使用化学计量来有效地区分德克斯和莱万EPS.

关键词:
美国的FTIR是FTIR.莱万的果实可以吃.德克斯兰 (Dextran) 是一种在外聚糖类中,含有外聚糖类.机器学习是机器学习.糖甜菜 糖甜菜 糖甜菜甘糖的使用方法

更多相关视频

OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides
08:43

OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides

Published on: June 20, 2010

13.8K
High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
11:31

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

Published on: September 15, 2015

10.1K

相关实验视频

Last Updated: Sep 11, 2025

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

11.6K
OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides
08:43

OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides

Published on: June 20, 2010

13.8K
High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
11:31

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

Published on: September 15, 2015

10.1K

科学领域:

  • 农业科学 农业科学
  • 生物技术是生物技术.
  • 分析化学 分析化学

背景情况:

  • 从消耗糖的微生物中获得的微生物衍生的外聚糖 (EPS) 在原糖制造中带来了重大运营挑战.
  • 这些EPS,主要是德克斯和果糖,导致工厂的损失,需要改进表征方法.
  • 开发有效的方法来识别和管理EPS对于优化糖生产过程至关重要.

研究的目的:

  • 开发和验证一种可访问的分析方法,用于在糖作物加工中对外聚糖 (EPS) 的表征.
  • 为了区分德克斯和莱文果糖,这两种主要类型的EPS影响原糖生产.
  • 为改善糖业中EPS的工程实践和管理策略提供基础.

主要方法:

  • 从糖加工设施中分离出来的细菌菌株被用于生产EPS.
  • 福里埃变换红外光谱法 (FTIR) 用于分析EPS样品和标准.
  • 化学测量技术,包括主要成分分析 (PCA) 和机器学习算法,用于数据分析和分类.

主要成果:

  • 应用于FTIR数据的主要成分分析 (PCA) 成功区分了德克斯和莱文EPS.
  • 机器学习技术 (LDA,SVM,k-NN) 根据FTIR光谱数据有效地对EPS进行了分类.
  • 基因组分析证实了在分离的细菌菌株中存在产生德克斯和莱文的基因,证实了光谱发现.

结论:

  • 该研究展示了在原糖生产中用于EPS的具有成本效益和低劳动力的分析分类方法.
  • 这种使用FTIR光谱的化学测量方法有可能提高糖加工设施的EPS管理.
  • 未来的研究应该专注于将这些方法应用于糖作物果汁和混合微生物培养等复杂样品.