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通过直接静电复合与纤维素纳米球来增强lyszyme的物理化学稳定性和生物催化活性

Nur Khairun Atiyah Sagee Ahmad¹, Balqis Az-Zahraa Norizan¹, Doris Huai Xia Quay²

¹Department of Applied Physics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia.

Carbohydrate polymers

|August 16, 2025

概括

纤维素纳米球有效地稳定了lyszyme,增强了其酶活性和抗菌特性. 这项研究表明纤维素纳米球是改善酶递送和功能的有希望的载体.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
酵素工程是什么意思酵素工程

背景情况:

酶稳定性对于有效的应用至关重要.
开发像酶这样的酶的有效载体是一个持续的挑战.
纤维素纳米晶 (CNC) 为生物材料开发提供了潜力.

研究的目的:

合成纤维素纳米圈 (CNS) 作为酶 (Lys) 的载体.
评估在中枢神经系统中封装的酶的稳定性和生物活性.
评估中枢神经系统/lys复合体的抗菌疗效.

主要方法:

控制注入油中的水 (W/O) 乳液方法,使用来自棉花的CNC.
通过FTIR,TEM和zeta潜力 (ZP) 进行中枢神经系统和中枢神经系统/lys复合体的表征.
在不同温度,pH值和尿素度下进行酶活性测定;针对Bacillus subtilis的抗菌测定.

主要成果:

最佳中枢神经系统 (3CNS11) 的粒子大小为242nm,PDI为0.4,ZP为-35.27mV.
通过光谱和显微镜证实了稳定的中枢神经系统/lys复合体的成功形成.
中枢神经系统/lys表现出增强的酶活性 (在70°C时为35%,在pH3时为60%) 并保留了尿素>80%的活性.
与自由酶相比,中枢神经系统/lys 对B. subtilis的抗菌抑制区域比自由酶的抗菌抑制区域大25%.

结论:

纤维素纳米球增强了酶的稳定性和生物活性.
中枢神经系统是提高酶性能的有效载体.
开发的CNS/Lys系统显示出需要增强酶稳定性和抗菌活性的应用潜力.

关键词:

抗菌剂抗菌剂抗菌剂 B. subtilis 的意思,就是说.运营商运营商运营商运营商这是一种乳液乳液.酵素酶是一种酶.排斥力排斥力排斥力

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