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在沉浸液体培养中,Bacillus paralicheniformis TB197的热和剪压诱导的表型变化

Estefany Chavarria-Quicaño^1,2, Miguel A Hernández-Oñate³, Jorge A Rodríguez-González⁴

¹Department of Food Science, Centro de Investigación en Alimentación y Desarrollo, Hermosillo, México.

Applied microbiology and biotechnology

|August 16, 2025

概括

热量和剪切压力对Bacillus paralicheniformis TB197的生长和脂质 (LP) 的产生产生影响. 最佳的LP生物合成发生在40°C和600rpm,而更高的温度会诱导分泌和生物膜的形成.

科学领域:

微生物学微生物学
生物技术是生物技术.
生物化学生化学

背景情况:

细菌种类产生的脂 (LPs),有价值的工业化合物通过定数感应调节.
包括热量和剪切在内的非生物应力可以影响LP合成和细菌表型.

研究的目的:

为了研究热和剪切应激对Bacillus paralicheniformis TB197生长,LP生产和表型表达的影响.
为了确定LP生物合成的最佳条件,以应对受控的压力.

主要方法:

对Bacillus paralicheniformis TB197的受控浸泡培养被置于不同的温度 (30°C,40°C,45°C) 和振动速度 (300-600rpm) 中.
分析了细胞度,LP产生和基因表达 (tasA, hag, sspB, fenB, lichA).
用差异表达分析来评估基因调节.

主要成果:

在30°C和40°C时增加 (600rpm) 显著提高细胞度和LP产量.
B和lichA的最佳LP产生和上调发生在40°C和每分钟600转时.
在45°C时,LP的产生减少,并增加了分泌基因 (sspB) 和生物膜 (tasA) 基因的表达.

结论:

热和机械应力差异调节B. paralicheniformis TB197表型和LP生产.
40°C和600rpm的条件促进LP产生表型,并优化生物合成.
压力条件可以抑制运动性,同时促进胞和生物膜的形成.

关键词:

细菌细胞表型的表型生物膜是一种生物膜.旗 (Flagella) 是一个旗.脂是一种脂.子子子

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